Isothermal Titration Calorimetric Studies on the Binding of a Family 6 Carbohydrate-binding Module of Clostridium thermocellum XynA with Xlylooligosaccharides(Biochemistry & Molecular Biology)

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概要

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• The family 6 carbohydrate-binding module (CBM) of Clostridium thermocellum XynA was expressed, and the binding equilibria of the CBM with xylooligosaccharides (degree of polymerization DP = 2-8) were observed by isothermal titration calorimetry (ITC) at pH 8. The association constant, K_a, increased with increasing DP from 5 × 10^3 M^<-1> (DP = 2) to approximately 5 × 10^5 M^<-1> (DP = 5-8) at 20℃. The K_a values at 60℃ were about 1/10 of those at 20℃. The binding was found to be an enthalpy-driven reaction. The DP dependence of the thermodynamic parameters of the binding reaction suggested the size of the ligand-binding site to be 5 xylose units long.

• 社団法人日本農芸化学会の論文
• 2003-02-23

著者

• Ohmiya K

  Graduate School Of Bioresources Mie Univ.

• Ohmiya Kunio

  Faculty Of Bioresources Mie University

• Araki T

  Faculty Of Bioresources Mie University

• Arai Takamitsu

  Faculty Of Bioresources And Center For Molecular Biology And Genetics Mie University

• Tanaka Akiyoshi

  Faculty of Bioresources, Mie University

• KIMURA Tetsuya

  Faculty of Bioresources, Mie University

• Kajino T

  Special Research Laboratory Ii Toyota Central R&d Laboratories Inc.

• Tanaka A

  Faculty Of Bioresources Mie University

• Aburatani Takeshi

  Faculty Of Bioresources Mie University

• SAKKA Kazuo

  Faculty of Bioresources, Mie University

• NAKANISHI Mari

  Faculty of Bioresources, Mie University

• SOGABE Mari

  Faculty of Bioresources, Mie University

• OHARA Hiroki

  Faculty of Bioresources, Mie University

• Sogabe Mari

  Faculty Of Bioresources Mie University

• Sakka Kazuo

  Faculty Of Bioresources Mie University

• Ohara Hiroki

  Faculty Of Bioresources Mie University:(present Address)meiji Seiko Kaisha Ltd.

• Arai T

  National Inst. Of Fruit Tree Sci. Nagasaki Jpn

• Nakanishi Mari

  Faculty Of Bioresources Mie University

• Kimura Tetsuya

  Graduate School Of Bioresources Mie University

• Tanaka Akiyoshi

  Faculty Of Bioresources Mie University

• Kimura Tetsuya

  Faculty Of Bioresources Mie University

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