Sequence Analysis and Overexpression of a Pectin Lyase Gene (pel1) from Aspergillus oryzae KBN616

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概要

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• A gene (pel1) encoding pectin lyase (Pel1) was isolated from a shoyu koji mold, Aspergillus oryzae KBN616,and characterized. The structural gene comprised 1,196 bp with a single intron. The ORF encoded 381 amino acids with a signal peptide of 20 amino acids. The deduced amino acid sequence showed high similarity tt those of Aspergillus niger pectin lyases and Glomerella cingulata PnlA. The pel1 gene was successfully overexpressed under the promoter of the A. oryzae TEF1 gene. The molecular mass of the recombinant pectin lyase substantially coincided with that calculated based on nucleotide sequence.

• 社団法人日本農芸化学会の論文
• 2001-01-23

著者

• Ohmiya Kunio

  Faculty Of Bioresources Mie University

• Kitamoto Noriyuki

  Food Research Center, Aichi Industrial Technology Institute

• TSUKAGOSHI Norihiro

  Department of Food Science and Technology, Faculty of Agriculture, Nagoya University

• Yoshino Shoko

  Food Research Institute Aichi Prefectural Government

• YOSHINO-YASUDA SHOKO

  Food Research lnstitute, Aichi Prefectural Government

• Kitamoto N

  Food Research Center Aichi Industrial Technology Institute

• Kitamoto Noriyuki

  Food Research Center Aichi Industrial Technology Institute

• Tsukagoshi N

  Department Of Biological Mechanisms And Functions Graduate School Of Bioagricultural Sciences Nagoya

• Tsukagoshi Norihiro

  Department Of Applied Biological Sciences Faculty Of Agriculture Nagoya University

• Kitamoto Noriyuki

  Food Res. Center Aichi Ind. Tech. Inst.

• YOSHINO-YASUDA Shoko

  Food Research Center, Aichi Center for Industry and Science Technology

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