Production of Polygalacturonase by Recombinant Aspergillus oryzae in Solid-State Fermentation Using Potato Pulp
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概要
- 論文の詳細を見る
The aim of this study was to produce valuable products from potato pulp, which is a byproduct of potato starch production and contains a high concentration of starch. We used the pulp as a substrate for polygalacturonase (PG) production in solid-state fermentation by a recombinant Aspergillus oryzae strain, PGB3. To generate PGB3, we constructed the PG gene (pgaB) overexpression vector pGBmR, and used that vector to transform the A. oryzae strain, RIB40. PGB3 carries a PG gene under control of the Taka-amylase gene promoter. When cultured in potato pulp solid medium, PGB3 grew more rapidly and produced 4.5 times more PG than RIB40. We observed maximum production (173 U/g) of PG after 2 days of culture of PGB3 in potato pulp solid medium. This high-level PG production from potato pulp demonstrates its potential as a useful substrate in fermentation.
- 社団法人 日本食品科学工学会の論文
- 2010-09-01
著者
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Kitamoto Noriyuki
Food Research Center, Aichi Industrial Technology Institute
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Hattori Ryota
National Food Research Institute, National Agriculture and Food Research Organization (NARO)
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TADA Sawaki
National Food Research Institute, National Agriculture and Food Research Organization (NARO)
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SUZUKI Satoshi
National Food Research Institute, National Agriculture and Food Research Organization (NARO)
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KUSUMOTO Ken-Ichi
National Food Research Institute, National Agriculture and Food Research Organization (NARO)
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Kusumoto K
National Food Research Institute
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Tada Sawaki
National Food Research Institute
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Suzuki S
National Food Research Institute
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Fukuoka Mari
National Food Research Institute
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Fukuoka Mari
National Food Research Institute Naro
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Kuriyama Ken-ichi
Department Of Applied Molecular Biology And Biochemistry Tokyo University Of Agriculture And Technol
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MATSUSHITA MORITA
National Food Research Institute, NARO
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Hattori Ryota
National Food Research Institute National Agriculture And Food Research Organization
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Matsushita Mayumi
National Food Research Institute National Agriculture And Food Research Organization (naro)
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Kusumoto Ken-ichi
National Food Research Institute
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Kitamoto Noriyuki
Food Research Center Aichi Industrial Technology Institute
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Kitamoto Noriyuki
Aichi Industrial Technol. Inst. Aichi Jpn
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Suzuki Satoshi
National Food Research Institute Naro
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Suzuki Satoshi
National Food Research Institute
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Kitamoto Noriyuki
Food Res. Center Aichi Ind. Tech. Inst.
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Hattori Ryota
Applied Microbiology Division, National Food Research Institute
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