High-Throughput Screening for Isolation of Enhanced Green Fluorescent Protein Expressing Transformants of Filamentous Fungus Using an Image Analyzer(METHODS)
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概要
- 論文の詳細を見る
High-throughput screening of enhanced green fluorescent protein (EGFP) gene-trap transformants of filamentous fungus was achieved for the first time using an image analyzer to measure their fluorescent intensity. For quantitative analysis of EGFP fluorescent intensity per unit cell mass, we developed a method for measurement of cell mass using the fluorescent dye SP-Dil. This method offers an effective and convenient tool for screening transformants expressing fluorescent protein as a reporter, and for quantitative analysis of fungal cell mass at fig levels.
- 社団法人日本生物工学会の論文
- 2003-09-25
著者
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Kashiwagi Yutaka
National Food Research Institute
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SUZUKI Satoshi
National Food Research Institute, National Agriculture and Food Research Organization (NARO)
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KUSUMOTO Ken-Ichi
National Food Research Institute, National Agriculture and Food Research Organization (NARO)
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Kusumoto K
National Food Research Institute
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Suzuki S
National Food Research Institute
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Taketani Hiroko
National Food Research Institute
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TEKETANI HIROKO
National Food Research Institute
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Kuriyama Ken-ichi
Department Of Applied Molecular Biology And Biochemistry Tokyo University Of Agriculture And Technol
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KUSUMOTO Kenichi
National Food Research Institute
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Kusumoto Ken-ichi
National Food Research Institute
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Kashiwagi Y
National Food Research Institute
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Suzuki Satoshi
National Food Research Institute Naro
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Suzuki Satoshi
National Food Research Institute
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