Kinetic Studies on p-Nitrophenyl-cellobioside Hydrolyzing Xylanase from Cellvibrio gilvus
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概要
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Reactions of the p-nitrophenyl-cellobioside (G_2-pNP) hydrolyzing xylanase from Cellvibrio gilvus (XCEL) were investigated kinetically in detail. XCEL hydrolyzed only aglyconic bonds in various arylcellobiosides with close kinetic paramters together. Its kinetic parameters toward various p-nitrophenyl cellooligosaccharides were also close. Two more xylanases, from Streptomyces sp. E86 (XSTR) and from Aspergillus japonicus (XASP), were found to hydrolyze G_2-pNP at a lower rate compared with XCEL. The V_<max> of XSTR and XASP were comparable to that of XCEL, suggesting that the high G_2-pNP-hydrolyzing activity of XCEL was due to its small K_m. A xylanse from Robillarda sp. Y-20 (XROB) did not have any activity on G_2-pNP. p-Nitrophenyl-xylobioside (X_2-pNP) and p-nitrophenyl-glucosyl-xyloside (GX-pNP) were examined as substrates to the four xylanases. Three of the four xylanases hydrolyzed these substrates, only at their aglyconic bonds, rather faster than xylan, but XROB hydrolyzed them with a very small rate. Classification of xylanases based on their activity on the aryl-glycosides is discussed. The advantage of using X_2-pNP or GX-pNP for xylanase assay is also discussed.
- 社団法人日本農芸化学会の論文
- 1993-12-23
著者
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Kitaoka Motomitsu
National Food Research Institute, National Agriculture and Food Research Organization
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Kusakabe Isao
Institute of Applied Biochemistry, University of Tsukuba
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Sawa T
Institute Of Microbial Chemistry
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Kitaoka M
Enzyme Laboratory National Food Research Institute
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Kitaoka M
Chubu Univ. Aichi Jpn
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Kitaoka Motomitsu
National Food Res. Inst.
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Kitaoka Motomitsu
Enzyme Laboratory National Food Research Institute National Agriculture And Food Research Organizati
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Haga Keiko
National Food Research Institute
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Kashiwagi Yutaka
National Food Research Institute
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Sasaki Takashi
National Food Research Institute
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Taniguchi Hajime
National Food Research Institute
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Kitaoka Motomitsu
Department Of Biological Chemistry Chubu University
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Kusakabe Isao
Institute Of Applied Biochemistry University Of Tsukuba
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Taniguchi H
Yamaguchi Univ. Yamaguchi Jpn
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Kashiwagi Y
National Food Research Institute
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Kitaoka Motomitsu
National Agriculture And Food Research Organization National Food Research Institute
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Taniguchi Hajime
National Food Research Insitute, Ministry of Agricultuer, Forestry and Fisheries
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