Purification and Characterization of Recombinant Mortierella vinacea α-Galactosidases I and II Expressed in Saccharomyces cerevisiae

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概要

• 論文の詳細を見る

• The cDNAs coding for Mortierella vinacea α-galactosidases I and II were expressed in Saccharomyces cerevisiae under the control of the yeast GAL10 promoter. The recombinant enzymes purified to homogeneity from the culture filtrate were glycosylated, and had properties identical to those of the native enzymes except for improving the heat stability of α-galactosidase II and decreasing the specific activities of both enzymes.

• 社団法人日本農芸化学会の論文
• 1999-06-23

著者

• KOBAYASHI Hideyuki

  National Astronomical Observatory of Japan

• Kobayashi H

  National Food Research Institute

• Kusakabe Isao

  Institute of Applied Biochemistry, University of Tsukuba

• YOSHIDA Shigeki

  Institute of Applied Biochemistry, University of Tsukuba

• KANEKO Satoshi

  Food Biotechnology Division, National Food Research Institute

• Kaneko Satoshi

  Food Biotechnology Div. National Food Res. Inst.

• KANEKO Satoshi

  National Food Research Institute

• Park G

  Institute Of Applied Biochemistry University Of Tsukuba

• Kobayashi H

  National Food Res. Inst. Ibaraki Jpn

• Yoshida S

  Plant Science Center Riken Institute

• Shibuya Hajime

  Institute Of Applied Biochemistry University Of Tsukuba

• Yoshida S

  Cancer Immunotherapy Center Nagoya Kyoritu Hospital

• Yoshida S

  Laboratory Of Cancer Cell Biology Research Institute For Disease Mechanism And Control Nagoya Univer

• Kiuchi Hiroshi

  Research Development Section Kibun Food Chemifa Co. Ltd.

• Hayashi K

  National Food Research Institute Ministry Of Agriculture Forestry And Fisheries

• Kaneko Satoshi

  National Food Res. Inst.

• Kaneko S

  Food Biotechnology Division National Food Research Institute

• Suzuki Y

  Department Of Applied Biological Chemistry The University Of Tokyo

• Park Gun

  Department Of Food Engineering And Biotechnology Kyungwon University

• PARK GwiGun

  Department of Food Engineering and Biotechnology, Kyungwon University

• Yoshida Shigeki

  Inst. Applied Biochem. Tsukuba Univ.

• Kaneko Shigenobu

  National Institute Of Crop Science (nics)

• Suzuki Yoshihito

  Department Of Applied Biological Chemistry The University Of Tokyo

• Suzuki Y

  Grad. Sch. Bioagric. Sci. Nagoya Univ.

• Hayashi K

  Department Of Biochemical Sciences Kagoshima University

• Kusakabe I

  Univ. Tsukuba Tsukuba Jpn

• Kusakabe Isao

  Institute Of Applied Biochemistry University Of Tsukuba

• 澁谷 源

  森林総研

• Yoshida Shigeki

  Institute Of Applied Biochemistry University Of Tsukuba

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