Cloning and Sequence Analysis of cDNA for Irpex lacteus Aspartic Proteinase(Biological Chemistry)
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概要
- 論文の詳細を見る
To find the primary sequence of Irpex lacteus aspartic proteinase (ILAP), a cDNA library of I. lacteus mRNA in pBR322 was constructed. A clone, which had an insert size of about 1.2 kilobase pairs, was found to contain the coding region of the mature enzyme. The deduced amino acid sequence showed that the enzyme consisted of 340 amino acid residues with a molecular weight of 35,000. Cysteine and methionine were not found in the enzyme and two putative N-glycosylation sites were indicated. The lack of S-S bridges in the molecule is a striking feature of the enzyme. The alignment of the sequence of the enzyme against other aspartic proteinases revealed homology around the active site aspartic acid residues.
- 社団法人日本農芸化学会の論文
- 1989-07-23
著者
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Murakami K
Research And Development Department Kibun Food Chemifa Co. Ltd.
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Kobayashi H
National Food Research Institute
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Kusakabe Isao
Institute of Applied Biochemistry, University of Tsukuba
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Murakami Kazuo
Institute of Applied Biochemistry, University of Tsukuba
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YOSHIDA Shigeki
Institute of Applied Biochemistry, University of Tsukuba
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KOBAYASHI Hideyuki
Institute of Applied Biochemistry, University of Tsukuba
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SEKIBATA Satoshi
Institute of Applied Biochemistry, University of Tsukuba
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SHIBUYA Hiroshi
Institute of Applied Biochemistry, University of Tsukuba
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Yoshida S
Plant Science Center Riken Institute
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Shibuya Hajime
Institute Of Applied Biochemistry University Of Tsukuba
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Kiuchi Hiroshi
Research Development Section Kibun Food Chemifa Co. Ltd.
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Hayashi K
National Food Research Institute Ministry Of Agriculture Forestry And Fisheries
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Murakami K
Univ. Tsukuba Ibaraki Jpn
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Yoshida Shigeki
Inst. Applied Biochem. Tsukuba Univ.
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Kusakabe I
Univ. Tsukuba Tsukuba Jpn
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Kusakabe Isao
Institute Of Applied Biochemistry University Of Tsukuba
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澁谷 源
森林総研
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Yoshida Shigeki
Institute Of Applied Biochemistry University Of Tsukuba
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Murakami Kazuo
Institute Of Applied Biochemistry University Of Tsukuba
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Kobayashi Hideyuki
Institute Of Applied Biochemistry The University Of Tsukuba
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Sekibata Satoshi
Institute Of Applied Biochemistry University Of Tsukuba
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