Purification and Characterization of Intracellular α-Glucuronidase from Aspergillus niger 5-16
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概要
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Two kinds of α-glucuronidases, CM-I and CM-II, were purified from a cell-free extract of Aspergillus niger 5-16. Molecular weights of both CM-I and CM-II were 130,000 by SDS-PAGE, and 150,000 by gel filtration. The optimum temperature and pH for the both enzyme reactions were 60℃ and 4.8. Both enzymes were stable up to 50℃, and between pHs 4.5 to 7.0 for 1 hr. Both enzymes were strongly inhibited by Ag^+, Hg^<2+>, Pb^<2+>, Sn^<2+>, Fe^<2+>, Fe^<3+>, Al^<3+>, sodium dodecyl sulfate, monoiodoacetic acid, and N-bromosuccinimide. The K_m of CM-I toward glucuronosyl-xylotriose and 4-O-methyl-glucuronosyl-xylotriose were 0.77 and 0.37,and those of CM-II were 0.82 and 0.47 mM, respectively. The V_<max> of CM-I toward glucuronosyl-xylotriose and 4-O-methyl-glucuronosyl-xylotriose were 5.20 and 1.42,and those of CM-II were 17.1 and 4.68 μmol of glucuronic acid formed/min/mg of protein, respectively.
- 社団法人日本農芸化学会の論文
- 1992-10-23
著者
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Murakami K
Research And Development Department Kibun Food Chemifa Co. Ltd.
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Kusakabe Isao
Institute of Applied Biochemistry, University of Tsukuba
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Murakami Kazuo
Institute of Applied Biochemistry, University of Tsukuba
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Kawabata Yasuyuki
Institute of Applied Biochemistry, University of Tsukuba
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UCHIDA HIROMI
Institute of Applied Biochemistry, University of Tsukuba
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Murakami K
Univ. Tsukuba Ibaraki Jpn
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Nanri Tomoko
Institute Of Applied Biochemistry University Of Tsukuba
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Kusakabe I
Univ. Tsukuba Tsukuba Jpn
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Koyama Y
National Inst. Biosci. & Human Technol. Tsukuba Science City Jpn
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Kusakabe Isao
Institute Of Applied Biochemistry University Of Tsukuba
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Uchida H
Univ. Tsukuba Ibaraki Jpn
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Kawabata Y
Univ. Tsukuba Ibaraki Jpn
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Kawabata Yasuyuki
Institute Of Applied Biochemistry University Of Tsukuba
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Murakami Kazuo
Institute Of Applied Biochemistry University Of Tsukuba
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Uchida Hiromi
Institute Of Applied Biochemistry University Of Tsukuba
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