Isolation of the Mouse <I>Ren-1</I>C Gene and Characterization of Renin Gene Expression in Both ES-D3 Cells and Their Parental Mouse Strain

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概要

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• Renin is the rate-limiting enzyme in the renin-angiotensin system, the only known biochemical cascade that produces the potent octapeptide effector molecule angiotensin II that controls, to a large degree, cardiovascular homeostasis and reproductive function in mammals. In the present study, we have cloned the <I>Ren-1</I>C gene from the C57BL/6 mouse and determined its genomic structure. In order to provide the molecular basis for the application of the gene targeting method to the renin gene, we also characterized the structure and expression of the renin gene in embryonic stem D-3 (ES-D3) cells and in its parental mouse strains, 129/Sv mouse. The <I>Ren-1</I>C gene is 12 kilobases long and is composed of 9 exons interrupted by 8 introns. Renin mRNA was undetectable in ES-D3 cells but was found to be highly expressed in the submandibular gland of the 129/Sv mouse, in addition to the kidney. Southern blot analysis reveals that ES-D3 cells carry the additional duplicated renin gene, <I> Ren-2</I>, consistent with the classification of the 129/Sv mouse as a two-renin gene strain.

• 日本繁殖生物学会の論文

著者

• SUGIYAMA Fumihiro

  Laboratory Animal Research Center

• Murakami Kazuo

  Institute of Applied Biochemistry, University of Tsukuba

• Fukamizu Akiyoshi

  Institute Of Applied Biochemistry And Tsukuba Advance Research Alliaance Center University Of Tsukub

• TANIMOTO Keiji

  Institute of Applied Biochemistry

• TAMURA Kouichi

  Institute of Applied Biochemistry

• FUKAMIZU Akiyoshi

  Institute of Applied Biochemistry

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