組換え抗原を用いたマウスパルボウイルス特異的なELISA法の開発

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概要

• 論文の詳細を見る

• Nucleotide sequcences of mouse parvovirus (MPV) isolate, named MPV/UT, and mouse minute virus (MMV) were analyzed and used for expressing recombinant proteins in E. coli. ELISA tests using recombinant major capsid protein (rVP2) and recombinant major non-structural protein (rNS1) as antigens were developed and their performance in serologic detection of rodent parvovirus infection was assessed. MPV-rVP2 and MMV-rVP2 ELISAs reacted specifically with anti-MPV and anti-MMV mouse sera, respectively. MMV-rNS1 antigen had a wide reaction range with antisera to rodent parvoviruses including MPV, MMV, Kilham rat virus (KRV) and H-1 virus. All mice oronasally infected with MPV were seropositive at 4 weeks post-infection in screening by ELISAs using MPV-rVP2 and MMV-rNS1 antigens, but were negative by conventional ELISA using whole MMV antigen. A contact transmission experiment revealed that transmission of MPV occurred up to 4 weeks post-infection, and all cage mates were seropositive in screening with MPV-rVP2 and MMV-rNS1 ELISAs. These results indicate that MPV-rVP2 and MMV-rVP2 are specific ELISA antigens which distinguish between MPV and MVM infection, while MMV-rNS1 antigen can be used in generic ELISA for a variety of rodent parvoviruses. The higher sensitivity of MPV-rVP2 ELISA than conventional ELISA for detecting seroconversion to MPV in oronasally infected mice as well as in cage mates suggests the usefulness of MPV-rVP2 ELISA in quarantine and microbiological monitoring of MPV infection in laboratory mice.

• 2006-04-01

著者

• SUGIYAMA Fumihiro

  Laboratory Animal Research Center

• YAGAMI Ken-ichi

  Laboratory Animal Research Center

• 杉山 文博

  Institute Of Basic Medical Sciences Laboratory Animal Resource Center University Of Tsukuba

• 杉山 文博

  久留米大学医学部附属動物実験センター

• KUNITA Satoshi

  Laboratory Animal Resource Center, University of Tsukuba

• CHAYA Miyuki

  Laboratory Animal Resource Center, University of Tsukuba

• HAGIWARA Kozue

  Laboratory Animal Resource Center, University of Tsukuba

• ISHIDA Tomoko

  ICLAS Monitoring Center, Central Institute for Experimental Animals

• TAKAKURA Akira

  ICLAS Monitoring Center, Central Institute for Experimental Animals

• SUGIMOTO Tatsuya

  Developmental Research Laboratories, Shionogi & Co., Ltd.

• ISEKI Hiroyoshi

  Laboratory Animal Resource Center, University of Tsukuba

• FUKE Kumiko

  Laboratory Animal Resource Center, University of Tsukuba

• Takakura A

  Iclas Monitoring Center Central Institute For Experimental Animals

• Fuke Kumiko

  Laboratory Animal Resource Center University Of Tsukuba

• Chaya Miyuki

  Laboratory Animal Resource Center University Of Tsukuba

• 高倉 彰

  財団法人実験動物中央研究所

• Takakura Akira

  Iclas Monitoring Center Central Institute For Experimental Animals

• Takakura Akira

  実験動物中央研究所 Iclasモニタリングセンター

• Hagiwara Kozue

  Laboratory Animal Resource Center University Of Tsukuba

• Kunita Satoshi

  筑波大学生命科学動物資源センター

• Kunita Satoshi

  Laboratory Animal Resource Center Tsukuba University

• Kunita Satoshi

  Laboratory Animal Resource Center University Of Tsukuba

• Kunita Satoshi

  実験動物中央研究所

• Kunita Satoshi

  Institute Of Experimental Animal Research School Of Science Kitasato University

• Ishida Tomoko

  Iclas Monitoring Center Central Institute For Experimental Animals

• Yagami Ken-ichi

  Laboratory Animal Resource Center University Of Tsukuba

• Yagami Ken-ichi

  Institute Of Basic Medical Sciences Laboratory Animal Resource Center University Of Tsukuba

• Iseki Hiroyoshi

  Laboratory Animal Resource Center University Of Tsukuba

• Sugimoto Tatsuya

  Developmental Research Laboratories Shionogi & Co. Ltd.

• Sugiyama Fumihiro

  Laboratory Animal Resource Center University Of Tsukuba

• 國田 智

  Laboratory Animal Resource Center, University of Tsukuba

• 茶谷 みゆき

  Laboratory Animal Resource Center, University of Tsukuba

• 萩原 梢

  Laboratory Animal Resource Center, University of Tsukuba

• Sugiyama Fumihiro

  Laborarory Animal Resource Center, University of Tsukuba, 1-1-1 Tennodai, Tsukuba 305-8575, Japan

• Yagami Ken-ichi

  Laborarory Animal Resource Center, University of Tsukuba, 1-1-1 Tennodai, Tsukuba 305-8575, Japan

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