Isolation and Identification Procedure for Staphylococcus aureus in Laboratory Mice and Rats by Combined Use of Chromogenic X-SA Agar and Specific Polymerase Chain Reaction(Laboratory Animal Science)
スポンサーリンク
概要
- 論文の詳細を見る
Generally, a conventional culture-based examination procedure (detection by egg-yolk salt agar and subsequent identification by phenotypic tests) for confirmation of the presence of S. aureus (SA) in laboratory mice and rats requires approximately 4 days. To improve the culture-based examination procedure for SA in terms of rapidity and reliability, combined use of chromogenic X-SA agar (XSA) and PCR using newly designed specific primers for SA (XSA-PCR) that can shorten the examination time (25.5hr) was compared with the conventional procedure for SA. In 425 samples from mice and rats, 193 suspected isolates were detected by egg-yolk salt agar (EYSA), and 216 suspected isolates were detected by XSA. In the subsequent identification, 189 of 193 suspected isolates detected by EYSA were identified as SA by phenotypic tests (97.9%), and all 216 suspected isolates detected by XSA were identified as SA by PCR (100%). All SA-positive samples by the conventional procedure were included in the SA-positive samples by XSA-PCR. As a result, XSA-PCR was superior to the conventional procedure in detection rate and identification rate of SA. Therefore, XSA-PCR appears to be an effective tool for examination of SA in laboratory mice and rats that improves precision and shortens the examination time.
- 社団法人日本獣医学会の論文
- 2009-01-25
著者
-
TAKAKURA Akira
ICLAS Monitoring Center, Central Institute for Experimental Animals
-
Itoh T
Iclas Monitoring Center Central Institute For Experimental Animals
-
Takakura A
Iclas Monitoring Center Central Institute For Experimental Animals
-
高倉 彰
財団法人実験動物中央研究所
-
Takakura Akira
Iclas Monitoring Center Central Institute For Experimental Animals
-
Takakura Akira
実験動物中央研究所 Iclasモニタリングセンター
-
Goto Kazuo
Iclas Monitoring Center Central Institute For Experimental Animals
-
ITOH Toshio
ICLAS Monitoring Center, Central Institute for Experimental Animals
-
HAYASHIMOTO Nobuhito
ICLAS Monitoring Center, Central Institute for Experimental Animals
-
GOTOH Kazuo
Central Institute for Experimental Animals
-
Itoh Toshio
Iclas Monitoring Center Central Institute For Experimental Animals
-
Goto Kazuo
Department Of Genetics International Council For Laboratory Animal Science Monitoring Center Central
-
Takashima Ikuo
Hokkaido Univ. Hokkaido Jpn
-
Hayashimoto Nobuhito
Iclas Monitoring Center Central Institute For Experimental Animals
関連論文
- Generation of congenic mouse strains by introducing the virus-resistant genes, Mx1 and Oas1b, of feral mouse-derived inbred strain MSM/Ms into the common strain C57BL/6J
- Characterization and epitope mapping of monoclonal antibodies to the nucleocapsid protein of severe acute respiratory syndrome coronavirus
- 組換え抗原を用いたマウスパルボウイルス特異的なELISA法の開発
- ICLASの Animal Quality Network Program に基づく Performance Evaluation Program 試行結果 : ICLASから供給された被検物質を用いた微生物モニタリング方法の自己評価
- ペットのラットからのStreptobacillus moniliformisの分離(公衆衛生学)
- 実験感染および自然感染マウスからのマウスノロウイルス核酸の検出
- Pasteurella pneumotropica とV因子要求性 Pasteurella 科菌のF344-rnuラットを用いた感染実験
- ヒト-プロト型c-Ha-ras遺伝子導入無菌マウスを用いたHelicobacter pylori感染による胃癌誘発実験
- gyrB遺伝子配列を用いた実験動物由来 Pasteurella pneumotropica 分離株の系統樹解析
- Decontamination of Human Xenotransplantable Tumor with Mouse Hepatitis Virus by Implantation in Nude Rat: A Case Report
- Quarantine for Contaminated Pathogens in Transplantable Human Tumors or Infections in Tumor Bearing Mice
- 実験動物の微生物モニタリング用ELISAキットの開発
- A Solid-Phase Enzyme Immunoassay Using Guinea Pig C3 to Detect Anti-Mycoplasma pulmonis Antibody in the Sera of Infected Rats
- 酵素標識プロテインAを用いたELISA法によるマウスおよびモルモット血清中のセンダイウイルス抗体の検出〔英文〕
- マウス肝炎ウイルスとマウスアデノウイルスの抗体検索に用いるそれぞれ2種類の抗原株の検討
- 15種の病原体に対するマウス,ラットの抗体保有調査〔英文〕
- Construction of an infectious cDNA clone for Omsk hemorrhagic fever virus, and characterization of mutations in NS2A and NS5
- Genetic and antigenic analyses of a Puumala virus isolate as a potential vaccine strain
- Distribution and characterization of tick-borne encephalitis viruses from Siberia and far-eastern Asia
- Single point mutation in tick-borne encephalitis virus prM protein induces a reduction of virus particle secretion
- The Mechanism of Actinomycin D-Mediated Increase of Borna Disease Virus(BDV) RNA in Cells Persistently Infected by BDV
- Identification Procedure for Pasteurella Pneumotropica in Microbiologic Monitoring of Laboratory Animals
- Genotyping of Mouse Hepatitis Virus Strains by Restriction Endonuclease Analysis of Amplified Nucleocapsid Protein Genes
- Isolation and Identification Procedure for Staphylococcus aureus in Laboratory Mice and Rats by Combined Use of Chromogenic X-SA Agar and Specific Polymerase Chain Reaction(Laboratory Animal Science)
- Establishment of an Enzyme-Linked Immunosorbent Assay for Detection of Hantavirus Antibody of Rats Using a Recombinant of Nucleocapsid Protein Expressed in Escherichia coli
- Elimination of Pasteurella pneumotropica from a Contaminated Mouse Colony by Oral Administration of Enrofloxacin
- Comparison of Bacteriological, Genetic and Pathological Characters between Escherichia coli O115a,c:K(B) and Citrobacter rodentium
- Evaluation of PCR as a Means of Identification of Pasteurella pneumotropica
- Prevalence of "Orphan" Parvovirus Infections in Mice and Rats
- Detection of Cilia-Associated Respiratory Bacillus in Experimentally and Naturally Infected Mice and Rats by the Polymerase Chain Reaction
- Induction of Drug Metabolism-Related Enzymes by Methylcholanthrene and Phenobarbital in Transgenic Mice Carrying Human Prototype c-Ha-ras Gene and Their Wild Type Littermates
- Safety Test and Field Study of an Oil-Adjuvanted H5N1 Avian Influenza Inactivated Vaccine
- Evaluation of the potency, optimal antigen level and lasting immunity of inactivated avian influenza vaccine prepared from H5N1 virus
- Characteristic karyotype abnormalities including Robertsonian translocations found in eight mouse embryonic stem cell lines
- O-19. Chromosomal analysis of common marmoset embryonic stem cells(Abstracts for the oral and the poster presentations,Abstracts of the Joint Meeting: the 58th Annual Meeting of the Society of Chromosome Research and the 17th Annual Meeting of the Chromos
- Vertical Transmission to Embryo and Fetus in Maternal Infection with Rat Virus (RV)
- Establishment of a neutralization test involving reporter gene-expressing virus-like particles of tick-borne encephalitis virus
- Mode of Infection of Hokkaido Virus (Genus Hantavirus) among Grey Red-Backed Voles, Myodes rufocanus, in Hokkaido, Japan
- A comparative epidemiological study of hantavirus infection in Japan and Far East Russia
- Epizootiological and Epidemiological Study of Hantavirus Infection in Japan
- Comparison of virulence of various hantaviruses related to hemorrhagic fever with renal syndrome in newborn mouse model
- Epizootiological survey of hantavirus among rodent species in Ningxia Hui Autonomous Province, China
- LCMV Infection and Countermeasures Taken in Response
- Phenotypes of IRS-2 Deficient Mice Produced by Reproductive Technology are Stable
- O-18. Chromosomal analysis of mouse fertilized eggs after cryopreservation(Abstracts of the oral and poster presentations)
- O-22. Chromosomal abnormalities including Robertsonian translocations found in eight mouse embryonic stem (ES) cell lines(Abstracts of the oral and poster presentations, Abstract of the 56th Annual Meeting of The Society of Chromosome Research)
- Morphological and Sequence Analysis of Mycoplasma sp. Isolated from the Oral Cavity of a House Musk Shrew (Suncus murinus)
- Experimental Infection Studies of Pasteurella pneumotropica and V-factor Dependent Pasteurellaceae for F344-rnu Rats
- Viral envelope protein glycosylation is a molecular determinant of the neuroinvasiveness of the New York strain of West Nile virus
- Genetic and biological comparision of tick-borne encephalitis viruses from Hokkaido and Far-Eastern Russia
- Epidemiology of Tick-Borne Encephalitis (TBE) and Phylogenetic Analysis of TBE Viruses in Japan and Far Eastern russia
- Analysis of severe acute respiratory syndrome coronavirus structural proteins in virus-like particle assembly
- Puumala virus infection in Syrian hamsters (Mesocricetus auratus) resembling hantavirus infection in natural rodent hosts
- Characterization of Monoclonal Antibodies against Hokkaido Strain Tick-Borne Encephalitis Virus
- 免疫不全および免疫正常マウスに対するPasteurella pneumotropicaの病原性
- アイソレーター飼育はC57BL/6JJclマウスにインシュリン抵抗性を引き起こす
- 免疫不全および免疫正常マウスに対するPasteurella pneumotropicaの病原性
- Construction of a replicon and an infectious cDNA clone of the Sofjin strain of the Far-Eastern subtype of tick-borne encephalitis virus
- Current Microbiological Status of Laboratory Mice and Rats in Experimental Facilities in Japan
- Current Microbiological Status of Laboratory Mice and Rats in Experimental Facilities in Japan