Puumala virus infection in Syrian hamsters (Mesocricetus auratus) resembling hantavirus infection in natural rodent hosts
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概要
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The mechanism of hantavirus persistent infection in natural hosts is poorly understood due to a lack of laboratory animal models. Herein, we report that Syrian hamsters (Mesocricetus auratus) infected with Puumala virus (PUUV) at 4 weeks old show persistent infection without clinical symptoms for more than 2 months. IgG and IgM antibodies against the viral nucleocapsid protein and neutralizing antibody were first detectable at 14 days postinoculation (dpi) and maintained through 70 dpi. Viral RNA was first detected from 3 dpi in lungs and blood clots, and was detected in all tissues tested at 7 dpi. The viral RNA persisted for at least 70 days in the lungs, kidney, spleen, heart, and brain. The highest level of RNA copies was observed at 14 dpi in the lungs. Slight inflammatory reactions were observed in the lungs, adrenal glands, and brain. Immunohistochemical analysis revealed that PUUV antigen persisted until 56 dpi in the kidneys and adrenal glands. Infected hamsters showed no body weight loss or clinical signs. These results indicate that PUUV infection in hamsters is quite similar to the hantavirus infection of natural host rodents.
著者
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有川 二郎
北海道大学医学部附属動物実験施設
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Itoh T
Iclas Monitoring Center Central Institute For Experimental Animals
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Kumada Hiromitsu
冲中記念成人病研究所
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Kumada Hiromitsu
国家公務員共済組合連合会虎の門病院 肝臓科
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Kumada Hiromitsu
国家公務員共済組合連合会虎の門病院 肝臓内科
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Kariwa Hiroaki
Hokkaido Univ. Sapporo Jpn
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Kariwa Hiroaki
北海道大学 獣医研究科
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有川 二郎
北海道大学 大学院医学研究科微生物学講座
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Kumada H
Department Of Hepatology Toranomon Hospital
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ARIKAWA Jiro
Hokkaido University School of Medicine
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Arikawa Jiro
ベトナム
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Jiro Arikawa
Institute For Animal Experimentation School Of Medicine Hokkaido University
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Arikawa Jiro
Institute For Animal Experimentation School Of Medicine Hokkaido University
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Arikawa Jiro
Institute For Animal Experimentation Hokkaido University Graduate School Of Medicine
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Tanikawa Yoich
Graduate School Of Veterinary Medicine Hokkaido University
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Takashima I
Hokkaido Univ. Sapporo Jpn
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Takashima Ikuo
Hokkaido Univ. Hokkaido Jpn
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Arikawa J
Inst. For Animal Experimentation Hokkaido Univ. Graduate School Of Medicine
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Koga Hiroyuki
Department Of Hepatology Toranomon Hospital
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Kariwa H
Hokkaido Univ. Sapporo Jpn
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