Comparison of virulence of various hantaviruses related to hemorrhagic fever with renal syndrome in newborn mouse model

概要

論文の詳細を見る
The virulence of hantaviruses that are antigenically related but have different genetic characteristics from the prototype of hantavirus, Hantaan (HTN) virus, was examined in newborn mice. The H5 and B78 strains of the Amur (AMR) genotype, the Bao14 strain of the Far East (FE) genotype, and the 76-118 strain of HTN virus were inoculated subcutaneously (1 focus-forming unit; FFU) into newborn mice. All of the AMR and FE genotype viruses inoculated mice were died by 16 days post-infection (dpi) and 21 dpi, respectively, while 50％ of the HTN virus inoculated mice survived unti1 30 dpi. The AMR and FE genotype viruses inoculated mice had high viral titers in the lung (1.3x10⁶ to 1.3x10⁸ FFU/gram [g] tissue), brain(2.1x10⁷ to 1.2x10⁹ FFU/g tissue), and kidney(2.5x10⁵ to 1.6x10⁷ FFU/g tissue), and showed a detectable level of antibodies (titers 1:16-1:32) at 14 dpi. In contrast, the HTN virus infected mice had viruses only in the lungs at low titers (1.1-5.3x10⁵ FFU/g tissue). Observations of body-weight changes revealed that the AMR and FE genotype viruses inoculated mice had lower growth rates than the HTN virus inoculated mice. These data suggest that the AMR and FE genotype viruses are more virulent than the HTN virus in newborn mice.
北海道大学の論文
2004-02-27