EFFECT OF NEUTRALIZING MONOCLONAL ANTIBODIES ON HANTAAN VIRUS INFECTION OF THE MACROPHAGE P388D1 CELL LINE
スポンサーリンク
概要
- 論文の詳細を見る
The effect of neutralizing monoclonal antibodies (N MAbs) on Hantaan virus infection of macrophages was investigated using P388D1 cells, a murine macrophage cell line. MAbs to the G1 protein (G1b) and the G2 protein (G2a and G2c) neutralized viral infectivity in P388D1 cells. N MAbs to G1b showed much higher neutralizing potency than those to G2a and G2c. With each N MAbs, two distinct effects were observed : neutralization of viral infectivity occurring at high concentrations and enhancement of that at low concentrations. Non-neutralizing MAbs, on the other hand, showed only enhancement of viral infectivity even at high concentrations without any inhibitory effects. The Fab fragments of N MAbs showed neither neutralizing nor enhancing activities. However, when the virus-Fab complexes were reacted with the anti-Fab antibodies, both neutralization and enhancement of viral infectivity were restored depending on the dose of Fab fragments. These results indicate that Hantaan virus infection of P388D1 cells is mediated by the Fc portion of the antibodies and neutralization is dependent on the concentration of N antibodies bound bivalently to the neutralization site on the virion.
- 北海道大学の論文
- 1992-09-30
著者
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橋本 信夫
北大獣医公衆衛生
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橋本 信夫
北海道大獣医学部公衆衛生学
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Hashimoto N
Hokkaido Univ. Hokkaido Jpn
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有川 二郎
北海道大学医学部附属動物実験施設
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HASHIMOTO Nobuo
Department of Neurosurgery, Kyoto University Graduate School of Medicine
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Kariwa Hiroaki
Department Of Veterinary Public Health Faculty Of Veterinary Medicine Hokkaido University
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Yao Jian-sheng
Department Of Veterinary Public Health Faculty Of Veterinary Medicine Hokkaido University
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TAKASHIMA Ikuo
Department of Public Health, Faculty of Veterinary Medicine, Hokkaido University
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ARIKAWA Jiro
Institute of Immunological Science, Hokkaido University
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YOSHIMATSU Kumiko
Institute of Immunological Science, Hokkaido University
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有川 二郎
北海道大学 大学院医学研究科微生物学講座
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Hashimoto Nobuo
Department Of Veterinary Public Health Faculty Of Veterinary Medicine Hokkaido University
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ARIKAWA Jiro
Hokkaido University School of Medicine
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Arikawa Jiro
ベトナム
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Arikawa Jiro
Institute For Animal Experimentation School Of Medicine Hokkaido University
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Arikawa Jiro
Institute For Animal Experimentation Hokkaido University Graduate School Of Medicine
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Hashimoto N
Institute For Experimental Animals School Of Medicine Kanazawa Univ.
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Hashimoto Nobuo
Department Of Cerebrovascular Surgery National Cordiovascular Center
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Arikawa J
Inst. For Animal Experimentation Hokkaido Univ. Graduate School Of Medicine
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Yoshimatsu Kumiko
Institute For Animal Experimentation Hokkaido University Graduate School Of Medicine
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