ANTIBODY ASSAY FOR JAPANESE ENCEPHALITIS VIRUS IN BOVINE SERUM BY ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA)
スポンサーリンク
概要
- 論文の詳細を見る
Enzyme-linked immunosorbent assay (ELISA) was applied for seroepidemiological study of Japanese encephalitis (JE) using bovine sera. ELISA antibody titers were four fold higher than neutralizing antibody titers. Minimum titer of positive ELISA antibody was set at a serum dilution of 1 : 40 to eliminate the non-specific reaction against normal mouse brain components in a test antigen. Antibody prevalence rates against JE virus in Kagoshima, an endemic area, were 26.0% in ELISA and 23.5% in the neutralization test. Meanwhile, sera from Hokkaido, a JE free area, had a prevalence rate of 4.3% by ELISA. Cross-reaction in ELISA between JE and Negishi virus was seen in bovine sera from Kagoshima and also in immune rabbit sera.
- 北海道大学の論文
- 1982-12-28
著者
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橋本 信夫
北大獣医公衆衛生
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HASHIMOTO Nobuo
Department of Neurosurgery, Kyoto University Graduate School of Medicine
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Miyata Keiji
Department Of Public Health Faculty Of Veterinary Medicine Hokkaido University
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Miyata Keiji
Department Of Veterinary Public Health Faculty Of Veterinary Medicine Hokkaido University
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UEDA Masayuki
Department of Veterinary Public Health Faculty of Veterinary Medicine Hokkaido University
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Hashimoto Nobuo
Department Of Cerebrovascular Surgery National Cordiovascular Center
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Ueda Masayuki
Department Of Public Health Faculty Of Veterinary Medicine Hokkaido University
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