NUCLEOTIDE SEQUENCE OF A GENE ENCODING A NEW GENUS SPECIFIC PROTEIN OF CHLAMYDIA PSITTACI
スポンサーリンク
概要
- 論文の詳細を見る
DNA fragment No. 13 from the C. psittaci pigeon strain, has been cloned in the plasmid pUC19. Hybridization analysis revealed that the fragment maintained a chlamydial common sequence. Furthermore, nucleotide seqencing identified two partial open reading frames (ORF), 675b. p. and 530b. p.. Expression of ORFs revealed that the second ORF encoded 25KD polypeptide, whereas the first ORF did not produce any antigenic product. The 25KD β-galactosidase fusion protein reacted strongly with chlamydia-specific antibodies elicited against a number of different chlamydial strains. Gene Bank analysis showed that this cloned gene is not highly homologous with chlamydia or other organisms for which nucleotide sequences have already been published. This 25KD polypeptide may be an additional genus-specific antigen of Chlamydiae.
- 北海道大学の論文
- 1991-12-26
著者
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HASHIMOTO Nobuo
Department of Neurosurgery, Kyoto University Graduate School of Medicine
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TAKASHIMA Ikuo
Department of Public Health, Faculty of Veterinary Medicine, Hokkaido University
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SATO CHIAKI
Department of Structural Biology,Biomolecular Engineering Research Institute
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Sato Chiaki
Department Of Veterinary Public Health Faculty Of Veterinary Medicine Hokkaido University
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Hashimoto N
Institute For Experimental Animals School Of Medicine Kanazawa Univ.
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Hashimoto Nobuo
Department Of Cerebrovascular Surgery National Cordiovascular Center
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KATUMATA Atushi
Institute for Chemical Research, Kyoto University
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Katumata Atushi
Institute For Chemical Research Kyoto University
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Sato Chiaki
Department Of Public Health Faculty Of Veterinary Medicine Hokkaido University
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