LABELED AVIDIN-BIOTIN ENZYME-LINKED IMMUNOSORBENT ASSAY (LAB-ELISA) FOR DETECTION OF JAPANESE ENCEPHALITIS ANTIBODY IN SWINE SERA
スポンサーリンク
概要
- 論文の詳細を見る
Labeled avidin-biotin (LAB) enzyme-linked immunosorbent assay (ELISA) was applied to detect Japanese encephalitis (JE) antibody in swine sera. Purified JaGAr-01 vaccine of JE, biotin labeled anti-swine Ig or IgM rabbit IgG conjugate and horseradish peroxidase (HRP) were employed in the assay. The μ chain specific anti-IgM conjugate could detect only IgM antibody, but the anti-Ig conjugate could detect both IgM and IgG antibodibes to JE. When 2-2'-azino di-[3-ethylbenzthiazoline sulphonic acid](ABTS) was used as a substrate to HRP, sensitivity of LAB-ELISA was essentially the same as that of ordinary ELISA, but approximately 20 times higher than that of hemagglutination inhibition (HI) test. LAB-ELISA titers with ABTS were approximately 5 times higher than those with 5-amino salcylic acid (5-AS). However, non-specific reactions of sera were about 4 times lower in LAB-ELISA than in ordinary ELISA.
- 北海道大学の論文
- 1984-04-28
著者
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橋本 信夫
北大獣医公衆衛生
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Hashimoto N
Hokkaido Univ. Hokkaido Jpn
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有川 二郎
北海道大学医学部附属動物実験施設
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HASHIMOTO Nobuo
Department of Neurosurgery, Kyoto University Graduate School of Medicine
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TAKASHIMA Ikuo
Department of Public Health, Faculty of Veterinary Medicine, Hokkaido University
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ARIKAWA Jiro
Department of Veterinary Public Health, Faculty of Veterinary Medicine, Hokkaido University
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OHKUBO Yukihiro
Department of Veterinary Public Health, Faculty of Veterinary Medicine, Hokkaido University
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CHANG Hsi-Chi
Department of Veterinary Public Health, Faculty of Veterinary Medicine, Hokkaido University
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有川 二郎
北海道大学 大学院医学研究科微生物学講座
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Hashimoto Nobuo
Department Of Veterinary Public Health Faculty Of Veterinary Medicine Hokkaido University
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ARIKAWA Jiro
Hokkaido University School of Medicine
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Arikawa Jiro
ベトナム
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Arikawa Jiro
Institute For Animal Experimentation School Of Medicine Hokkaido University
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Arikawa Jiro
Institute For Animal Experimentation Hokkaido University Graduate School Of Medicine
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Chang Hsi-chi
Department Of Veterinary Public Health Faculty Of Veterinary Medicine Hokkaido University
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Hashimoto Nobuo
Department Of Cerebrovascular Surgery National Cordiovascular Center
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Arikawa J
Inst. For Animal Experimentation Hokkaido Univ. Graduate School Of Medicine
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Ohkubo Yukihiro
Department Of Veterinary Public Health Faculty Of Veterinary Medicine Hokkaido University
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ARIKAWA Jiro
Department of Microbiology, Graduate School of Medicine, Hokkaido University
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