Establishment of an Enzyme-Linked Immunosorbent Assay for Detection of Hantavirus Antibody of Rats Using a Recombinant of Nucleocapsid Protein Expressed in Escherichia coli
スポンサーリンク
概要
- 論文の詳細を見る
A recombinant nucleocapsid protein of Hantaan virus (HTN) 76-118 strain expressed in E. coli was applied as a serodiagnostic antigen in an enzyme-linked immunosorbent assay (rHTN-ELISA) for detection of hantavirus antibody in rat sera. The sensitivity and specificity of the rHTN-ELISA were compared with those of the indirect immunofluoresent assay (IFA) using virus-infected cells. The sensitivity of rHTN-ELISA was similar to that of the IFA both in experimentally SR-11 infected rat and naturally infected rat sera. Sera showing a low antibody titer in IFA and suspected to be negative by other methods were also found to be negative in rHTN-ELISA. These results indicate that rHTN-ELISA is effective as a screening method for serodiagnosis of hantaviruses, because of its high sensitivity, specificity, safety and suitability for processing large number of samples.
- 社団法人 日本実験動物学会の論文
- 2003-01-01
著者
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有川 二郎
北海道大学医学部附属動物実験施設
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Itoh T
Iclas Monitoring Center Central Institute For Experimental Animals
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Takakura A
Iclas Monitoring Center Central Institute For Experimental Animals
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高倉 彰
財団法人実験動物中央研究所
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Takakura Akira
実験動物中央研究所 Iclasモニタリングセンター
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TAKAKURA Akira
Central Institute for Experimental Animals
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ITOH Toshio
Central Institute for Experimental Animals
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Goto Kazuo
Iclas Monitoring Center Central Institute For Experimental Animals
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TAKASHIMA Ikuo
Laboratory of Public Health, Department of Environmental Veterinary Sciences, Graduate School of Vet
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ARIKAWA Jiro
Institute of Immunological Science, Hokkaido University
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YOSHIMATSU Kumiko
Institute of Immunological Science, Hokkaido University
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有川 二郎
北海道大学 大学院医学研究科微生物学講座
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GOTO Kazuo
Central Institute for Experimental Animals
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Takashima Ikuo
Laboratory Of Public Health Department Of Environmental Veterinary Sciences Graduate School Of Veter
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ARIKAWA Jiro
Hokkaido University School of Medicine
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Arikawa Jiro
ベトナム
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Jiro Arikawa
Institute For Animal Experimentation School Of Medicine Hokkaido University
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Arikawa Jiro
Institute For Animal Experimentation School Of Medicine Hokkaido University
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Arikawa Jiro
Institute For Animal Experimentation Hokkaido University Graduate School Of Medicine
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GOTOH Kazuo
Central Institute for Experimental Animals
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Goto Kazuo
Department Of Genetics International Council For Laboratory Animal Science Monitoring Center Central
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Takashima I
Hokkaido Univ. Sapporo Jpn
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Takashima Ikuo
Hokkaido Univ. Hokkaido Jpn
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Arikawa J
Inst. For Animal Experimentation Hokkaido Univ. Graduate School Of Medicine
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Yoshimatsu Kumiko
Institute For Animal Experimentation Hokkaido University Graduate School Of Medicine
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