Experimental Evaluation of Cross-contamination between Cryotubes Containing Mouse 2-cell Embroyos and Murine Pathogens in Liquid Nitrogen Tanks
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概要
- 論文の詳細を見る
It has been suspected that embryos stored in liquid nitrogen tanks may become contaminated with murine pathogens, if some pathogens had been introduced to the tanks accidentally. To examine this, we stored tubes containing embryos with tubes containing mouse hepatitis virus (MHV) or Pasteurella pneumotropica in liquid nitrogen tanks and examined whether progeny mice derived from the embryos were contaminated with the pathogens or not. After storing for 6 months or 1 year the frozen embryos were thawed and implanted into the oviducts of pseudopregnant female mice, and the mice were bred in vinyl isolators. We could not detect serum antibodies to MHV and isolate Pasteurella pneumotropica in the progeny mice, suggesting that cross-contamination between tubes in a liquid nitrogen tank scarcely occurs.
- 社団法人 日本実験動物学会の論文
- 2003-01-01
著者
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ITOH Toshio
Central Institute for Experimental Animals
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Kyuwa Shigeru
Graduate School Of Agricultural And Life Sciences The University Of Tokyo
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NOGUCHI Kazuhiro
Division of Microbiology and Genetics
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URANO Toru
Division of Microbiology and Genetics
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NISHIKAWA Takaki
Center for Animal Resources and Development, Kumamoto University
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KANEKO Takehito
Center for Animal Resources and Development, Kumamoto University
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NAKASHIMA Tatsuyuki
Center for Animal Resources and Development, Kumamoto University
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KAWANO Kayo
Center for Animal Resources and Development, Kumamoto University
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NAKAMURA Naoko
Center for Animal Resources and Development, Kumamoto University
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NOGUCHI Kazuhirio
Center for Animal Resources and Development, Kumamoto University
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URANO Toru
Center for Animal Resources and Development, Kumamoto University
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NAKAGATA Naomi
Center for Animal Resources and Development, Kumamoto University
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Nakamura Naoko
Division Of Reproductive Engineering Center For Animal Resources And Development (card) Kumamoto Uni
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Urano Toru
Center For Animal Resources And Development Kumamoto University
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Nakagata N
Center For Animal Resources And Dev. Kumamoto Univ.
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Nakagata N
Kumamoto Univ. Kumamoto Jpn
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Nakagata Naomi
Center For Animal Resources And Dev. Kumamoto Univ.
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Noguchi Kazuhiro
Center For Animal Resources And Development Kumamoto University
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Kawano Kayo
Center For Animal Resources And Development Kumamoto University
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Kaneko Takehito
Division Of Reproductive Engineering Center For Animal Resources And Development (card) Kumamoto Uni
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Nishikawa Takaki
Center For Animal Resources And Development Kumamoto University
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Nishihara Takuji
Div. Of Biological Sci. Graduate School Of Biology-oriented Sci. And Technol.
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Kaneko Takehito
Center For Animal Resources And Dev. Kumamoto Univ.
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