Effect of Mouse Intestinal Bacteria on Incidence of Colorectal Tumors Induced by 1, 2-dimethylhydrazine Injection in Gnotobiotic Transgenic Mice Harboring Human Prototype c-Ha-ras Genes
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概要
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We produced transgenic (Tg) gnotobiotic (GB) mice carrying human prototype c-Ha-ras genes and compared the incidence of colorectal tumors induced by 1,2-dimethylhydrazine (DMH) injection. At 7 to 11 weeks of age, germfree (GF) CB6F1-Tg Hras2 mice were inoculated with various mouse fecal suspensions or mixtures of bacteria isolated from mouse feces. Three weeks after bacterial inoculation, DMH was administered by subcutaneous injection at 20 mg per kg body weight for 20 weeks. Mice were euthanized 5 weeks after the last injection to investigate the number of colorectal tumors. The incidence of colorectal tumors was high in both Tg- and non-Tg-GF mice (100%). In Tg-specific pathogen-free (SPF) mice and Tg-GB-4 mice associated with basic mouse flora consisting of Escherichia coli, lactobacilli, Bacteroides and clostridia, the incidence of colorectal tumors was as high as that in GF mice. In Tg-SPF mice, the tumor score was higher than in Tg-GF mice (p<0.01), but no colorectal tumors were detected in non-Tg groups of SPF, and the tumor incidence was remarkably low in non-Tg-GB-4 mice. The tumor incidence and score in Tg- and non-Tg-GB mice varied depending on the bacterial combination in their intestine. These results indicate that the presence of human c-Ha-ras genes and intestinal bacteria substantially modify colorectal tumorigenesis induced by DMH.
- 社団法人 日本実験動物学会の論文
著者
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ITOH Kikuji
Department of Veterinary Public Health, Graduate School of Agricultural and Life Sciences, Universit
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NAKAYAMA Hiroyuki
Department of Veterinary Pathology, Graduate School of Agricultural and Life Sciences, The Universit
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Nakayama Hiroyuki
Department Of Internal Medicine And Therapeutics Osaka University Graduate School Of Medicine
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Hioki Kyoji
Central Inst. For Experimental Animals
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Hioki K
Central Inst. Experimental Animals Kanagawa
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Hioki K
Central Inst. Of Experimental Animals Kawasaki Jpn
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Nomura T
Central Inst. For Experimental Animals
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Nomura Tatsuji
実験動物中央研究所
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Nomura Tatsuji
Central Institute For Experimental Animals
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ITOH Toshio
Central Institute for Experimental Animals
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Itoh Kikuji
Lab. Of Veterinary Public Health The Univ. Of Tokyo
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Itoh Kikuji
Laboratory Of Veterinary Public Health Graduate School Of Agriculture And Life Science The Universit
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Itoh T
Central Inst. For Experimental Animals
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MITSUOKA Tomotari
The Institute of Physical and Chemical Research
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Mitsuoka Tomotari
Department of Food Hygiene, Nippon Veterinary and Animal Science University
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Mitsuoka T
Nippon Veterinary And Animal Sci. Univ. Tokyo Jpn
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Mitsuoka T
Laboratory Of Veterinary Public Health
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Mitsuoka Tomotari
Department Of Food Technology Nippon Veterinary And Animal Science University
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Mitsuoka Tomotari
Department Of Biomedical Science Faculty Of Agriculture The University Of Tokyo
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NARUSHIMA Seiko
Department of Veterinary Public Health
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Mitsuoka Tomotari
Laboratory Of Veterinary Public Health
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Mitsuoka Tomotari
Frontier Research Program Laboratory For Intestinal Flora Riken:faculty Of Agriculture The Universit
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Itoh Kikuji
Dep. Of Veterinary Public Health Graduate School Of Agricultural And Life Sciences The Univ. Of Toky
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Itoh Kikuji
Department Of Veterinary Medicine Graduate School Of Agricultural And Life Sciences The University O
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Nakayama Hiroyuki
Laboratory Of Veterinary Pathology Graduate School Of Agriculture And Life Science The University Of
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Narushima S
Lab. Of Veterinary Public Health The Univ. Of Tokyo
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