Process of the Development of T-2 Toxin-induced Apoptosis in the Lymphoid Organs of Mice
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概要
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Female ICR:CD-1 mice orally treated with 10 mg/kg b.w. of T-2 toxin were killed at 1, 3, 6, 9, 12, 24 and 48 hr after treatment (HAT) and subjected to examination of the process of the development of T-2 toxin-induced apoptosis in the thymus and spleen. The early ultrastructural changes in lymphocytes characterized by shrinkage of the cell body and condensation of nuclear chromatin were detected at 3HAT in the thymus. The number of apoptotic lymphocytes observed by the in situ detection method for fragmented DNA increased drastically from 9 to 24 HAT in the thymus while it began to increase at 12 HAT in the spleen. The DNA ladder was first detected by agarose gel electrophoresis at 9 HAT and became clearer at 12 and 24 HAT in the thymus but was not clearly detected in the spleen throughout the observation period. Thus T-2 toxin-induced apoptosis developed earlier and was apparently severer in the thymus than in the spleen. Apoptois was first detected by electron microscopy, then by the in situ detection method for fragmented DNA, and finally by DNA agarose gel electrophoresis.
- 社団法人 日本実験動物学会の論文
著者
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土井 邦雄
残留農薬研究所
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Nakayama H
Department Of Veterinary Pathology Graduate School Of Agricultural And Life Sciences University Of T
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Doi Kunio
Department of Veterinary Pathology, Faculty of Agriculture, The University of Tokyo
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NAKAYAMA Hiroyuki
Department of Veterinary Pathology, Graduate School of Agricultural and Life Sciences, The Universit
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UETSUKA Koji
Department of Veterinary Pathology, Graduate School of Agricultural and Life Sciences, The Universit
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Nakayama H
Dep. Of Veterinary Pathology Graduate School Of Agricultural And Life Sciences The Univ. Of Tokyo
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Nakayama Hiroyuki
Department Of Internal Medicine And Therapeutics Osaka University Graduate School Of Medicine
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SHINOZUKA Junko
Depart.Vet.Pathol., Fac.Agriculture, Univ.Tokyo
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Shinozuka Junko
田辺三菱製薬
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Doi Kunio
日本生物科学研究所
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Doi Kunio
Department Of Applied Chemistry Nagoya Institute Of Technology
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Uetsuka Koji
Department Of Veterinary Pathology Faculty Of Agriculture The University Of Tokyo
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GUANMIN Li
Department of Veterinary Pathology, Faculty of Agriculture, The University of Tokyo
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Uetsuka K
Department Of Veterinary Pathology Faculty Of Agriculture The University Of Tokyo
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Li Guanmin
Depart.vet.pathol. Fac.agriculture Univ.tokyo
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Saegusa J
National Inst. Industrial Health Kawasaki Jpn
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Saegusa Junzo
National Institute Of Occupational Safety And Health
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Saegusa Junzo
Department Of Human Engineering National Institute Of Industrial Health
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Guanmin Li
Depart.Vet.Pathol., Fac.Agriculture, Univ.Tokyo
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