Characteristic Intestinal Microflora of Specific Pathogen-Free Mice Bred in Two Different Colonies and their Influence on Postnatal Murine Immunocyte Profiles

スポンサーリンク

概要

• 論文の詳細を見る

• Cecal microflora of BALB/c mice originating from two different SPF-breeding colonies were compared. The analysis of cultivable bacteria in the ceca showed significantly higher numbers of total bacteria in BALB/cCrSlc (SLC mice) than in BALB/cA Jcl (JCL mice) (p<0.05), which were mainly based on higher numbers and occurrence of Peptococaceae. Bifidobacteria were detected only in SLC mice. Feeding an oligosaccharide, raffinose, to the mice also induced different shifts in the composition of cecal microflora and the concentration of cecal organic acids. In the second experiment, hysterectomy-derived (HD) SLC mice were fostered to SPF lactating SLC mothers, or SPF lactating JCL mice, together with the mothers own natural birth (NB) pups in each isolator. HD mice fostered to SLC-mothers showed significantly higher percentages of T-cell receptor αβ cells expressing a CD8α homodimer (p<0.05) and a CD8αβ heterodimer (p<0.001) in the intraepithelial lymphocytes (IEL) compared with HD mice fostered to JCL-mothers. IEL profiles of HD mice corresponded well to those of NB mice that were breast-fed by the same mothers. Differences in the ratio of B220+cells to Thy1.2+cells in the splenocytes were also observed as a trend between both HD mice fostered to SLC or JCL mothers (p=0.06). These results suggest that postnatal colonization of various characteristic intestinal microflora derived from SPF-breeding colonies results in differences in development of lymphocyte populations in the intestinal and systemic organs of mice.

• 社団法人　日本実験動物学会の論文
• 2005-04-01

著者

• Aritsuka Tsutomu

  Research Center, Nippon Beet Sugar Mfg. Co., Ltd.

• ITOH Kikuji

  Department of Veterinary Public Health, Graduate School of Agricultural and Life Sciences, Universit

• Itoh Kikuji

  Lab. Of Veterinary Public Health The Univ. Of Tokyo

• Itoh Kikuji

  Laboratory Of Veterinary Public Health Graduate School Of Agriculture And Life Science The Universit

• KAMINOGAWA Shuichi

  Department of Food Science and Technology, College of Bioresource Sciences, Nihon University

• HACHIMURA Satoshi

  Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The U

• NAGURA Taizo

  Department of Applied Biological Chemistry, University of Tokyo

• Kaminogawa Shuichi

  Department Of Applied Biological Chemistry Division Of Agriculture And Agricultural Life Sciences Th

• Itoh Kikuji

  Dep. Of Veterinary Public Health Graduate School Of Agricultural And Life Sciences The Univ. Of Toky

• Itoh Kikuji

  Department Of Veterinary Medicine Graduate School Of Agricultural And Life Sciences The University O

• Habu S

  Division Of Host Defense Mechanism Department Of Immunology Tokai University School Of Medicine

• Kaminogawa S

  Dep. Of Food Sci. And Technol. Nihon Univ.

• Hachimura S

  Res. Center For Food Safety Graduate School Of Agricultural And Life Sciences The Univ. Of Tokyo

• Nagura Taizo

  Research Center Nippon Beet Sugar Mfg. Co.

• Kaminogawa Shuichi

  Department Of Food Science And Technology College Of Bioresource Sciences Nihon University

• Kaminogawa Shuichi

  Department Of Agricultural Chemistry Faculty Of Engineering The University Of Tokyo

• Hachimura Satoshi

  Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni

• Aritsuka Tsutomu

  Research Center Nippon Beet Sugar Mfg. Co.

• Hachimura Satoshi

  Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni

• Aritsuka Tsutomu

  Research Center Nippon Beet Sugar Mfg Co. Ltd.

関連論文

• One-pot conversion of levan prepared from Serratia levanicum NN to difructose anhydride IV by Arthrobacter nicotinovorans levan fructotransferase(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
• Effect of a commercial microbiological additive on beef manure compost in the composting process
• Establishment of Specific Pathogen-Free (SPF) Rat Colonies using Gnotobiotic Techniques
• Establishment of Specific Pathogen-Free Rabbit Colonies with Limited-Flora Rabbits Asociated with Conventional Rabbit Flora, and Monitoring of Their Cecal Flora
• Production of Ex-Germfree Rabbits for Establishment of Specific Pathogen-Free (SPF) Colonies
• Antigenicity of Modified β-Lactoglobulin Examined by Three Different Assays
• Limited effect of chromatin remodeling on D_β-to-J_β recombination in CD4^+CD8^+ thymocyte : implications for a new aspect in the regulation of TCR β gene recombination
• Bacteroides Induce Higher IgA Production Than Lactobacillus by Increasing Activation-Induced Cytidine Deaminase Expression in B Cells in Murine Peyer’s Patches
• The in vivo development of human T cells from CD34^+ cells in the murine thymic environment
• Ex vivo evidence for asymmetric tyrosine phosphorylation of ZAP-70 on double-positive thymocytes in the positive selection process
• Dietary Melibiose Regulates Th Cell Response and Enhances the Induction of Oral Tolerance
• Characteristic Intestinal Microflora of Specific Pathogen-Free Mice Bred in Two Different Colonies and their Influence on Postnatal Murine Immunocyte Profiles
• Industrial production of difructose anhydride III(DFA III) from crude inulin extracted from chicory roots using Arthrobacter sp. H65-7 fructosyltransferase(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
• DNA-Synthesis Stimulating Peptide from Bovine β-Casein(Biological Chemistry)
• DNA-Synthesis Stimulating Peptides from Human β-Casein(Biological Chemistry)
• Presence of Cell Growth Promoting Peptides in Chicken Egg White Extract(Food & Nutrition)
• Stimulatory Effect of Human Lactoferrin on DNA Synthesis in BALB/c 3T3 Cells
• サトウキビ抽出液のブタ免疫応答調節能(免疫学)
• CD45 can act as a negative regulator for the transition from early to late CD4^+ CD8^+ thymocytes
• Transcription and demethylation of TCR β gene initiate prior to the gene rearrangement in c-kit^+ thymocytes with CD3 expression: evidence of T cell commitment in the thymus
• Inhibitory Effect of Apple Pectin and Culture Condensate of Bifidobacterium Iongum of Colorectal Tumors Induced by 1,2-Dimethylhydrazine in Transgenic Mice Harboring Human Prototype c-Ha-ras Genes
• Effect of Mouse Intestinal Bacteria on Incidence of Colorectal Tumors Induced by 1, 2-dimethylhydrazine Injection in Gnotobiotic Transgenic Mice Harboring Human Prototype c-Ha-ras Genes
• Establishment of Specific Pathogen-Free Guinea-pig Colonies Using Limited-Flora Guinea-pigs Associated with Conventional Guinea-pig Flora, and Monitoring of their Cecal Flora
• Role of Olfaction in Food Preference as Evaluated in an Animal Model
• Apoptosis of Antigen-specific T Cells Induced by Oral Administration of Antigen : Comparison of Intestinal and Non-intestinal Immune Organs
• Strain Dependency of the Immunopotentiating Activity of Lactobacillus delbrueckii subsp. bulgaricus
• Carboxypeptidase Taq, a Thermostable Zinc Enzyme, from Thermus aquaticus YT-1 : Molecular Cloning, Sequencing, and Expression of the Encoding Gene in Escherichia coli
• Purification and Characterization of a Thermostable Carboxypeptidase (Carboxypeptidase Taq) from Thermus aquaticus YT-1
• Vaccination with an immunodominant peptide of bovine type II collagen induces an anti-TCR response, and modulates the onset and severity of collagen-induced arthritis
• Suppression of Collagen-Induced Arthritis in DBA/1J Mice by Preimmunization with House Dust Mite Extract
• Enhancing Effect of Interleukin-4 on the Secretion of Interferon-γ by α_-Casein-specific CD8^+ T Cells
• α_-Casein-specific CD8^+ T Cell Clone That Inhibits Its Own Interferon-γ Production by Producing Interleukin-10
• Amino Acid Residue Substitution at T-Cell Determinant-flanking Sites in β-Lactoglobulin Modulates Antigen Presentation to T Cells through Subtle Conformational Change(Food & Nutrition Science)
• In Vivo Macrophage-stimulation Activity of the Enzyme-degraded Water-soluble Polysaccharide Fraction from a Marine Alga (Gracilaria verrucosa)
• Macrophage Stimulation Activity of the Polysaccharide Fraction from a Marine Alga (Porphyra yezoensis):Structure-Function Relationships and Improved Solubility
• Activation of Murine Macrophages by Polysaccharide Fractions from Marine Algae (Porphyra yezoensis)
• Antigen Feeding Increases Frequency and Antigen-specific Proliferation Ability of Intraepithelial CD4^+ T Cells in αβ T Cell Receptor Transgenic Mice(Food & Nutrition Science)
• Antigen Presentation by Peyer's Patch Cells Can Induce both Th1- and Th2-type Responses Depending on Antigen Dosage, but a Different Cytokine Response Pattern from That of Spleen Cells(Food & Nutrition Science)
• Characterization of a Water-soluble Polysaccharide Fraction with Immunopotentiating Activity from Bifidobacterium adolescentis M101-4
• Screening for the Immunopotentiating Activity of Food Microorganisms and Enhancement of the Immune Response by Bifidobacterium adolescentis M101-4
• Strong Influence of the Processing of the Antigen on Negative Effects on T Cell Activation by Regions Outside the Determinant Area of Bovine α_-Casein
• Production and Characterization of Anti-human Insulin Antibodies in the Hen's Egg(Biological Chemistry)
• Structure of α_-Casein at an Oil/Water Interface: An Analytical Approach Using Immunochemical Methods(Biological Chemistry)
• Methionine Oxidation and Proline cis-trans Isomerization in α_-Casein on Emulsification with Oxidized Lipid(Biological Chemistry)
• In situ demonstration of intraepithelial lymphocyte adhesion to villus microvessels of the small intestine
• Analysis of Cytokine Producing Activity of Intestinal Intraepithelial T Cells from TCR β-Chain and δ-Chain Mutant Mice
• Cyclophosphamide-Induced Bacterial Translocation in Escherichia coli C25-Monoassociated Specific Pathogen-Free Mice
• An Improved DNA Isolation Method for Metagenomic Analysis of the Microbial Flora of the Human Intestine
• Evidence of GATA-3-dependent T_h2 commitment during the in vivo immune response
• Dietary Nucleotides Increase the Proportion of a TCRγδ^+ Subset of Intraepithelial Lymphocytes (IEL) AND IL-7 Production by Intestinal Epithelial Cells (IEC) : Implications for Modification of Cellular and Molecular Cross-talk between IEL and IEC by Dieta
• Expression and Secretion of Bovine β-Lactoglobulin in Saccharomyces cerevisiae(Food & Nutrition)
• Purification, Characterization, and Gene Cloning of Lysyl Aminoeptidase from Streptococcus thermophilus YRC001(Biochemistry & Molecular Biology)
• Food allergens and mucosal immune systems with special reference to recognition of food allergens by gut-associated lymphoid tissue
• Cloning and Nucleotide Sequencing of Genes Encoding Mn-Superoxide Dismutase and Class II Fumarase from Thermus aquaticus YT-1
• Cloning of Genes of the Aminopeptidase T Family from Thermus thermophilus HB8 and Bacillus stearothermophilus NCIB8924 : Apparent Similarity to the Leucyl Aminopeptidase Family
• Comparison of pH and Ionic Strength Dependence of Interactions between Monoclonal Antibodies and Bovineβ-Lactoglobulin
• Bifidobacterium suppresses IgE-mediated degranulation of rat basophilic leukemia (RBL-2H3) cells
• Inhibitory Effect of Lactobacillus gasseri TMC0356 and Lactobacillus GG on Enhanced Vascular Permeability of Nasal Mucosa in Experimental Allergic Rhinitis of Rats
• Evidence for down-regulation of highly expressed TCR by CD4 and CD45 on non-selected CD4^+CD8^+ thymocytes
• The positional effect of Eβ on Vβ genes of TCRβ chain in the ordered rearrangement and allelic exclusion
• Changes in Normal Vaginal Flora of African Green Monkeys (Cercopithecus aethiops) during the Menstrual Cycle
• Establishment of a Primary Culture Method for Mouse Intestinal Epithelial Cells by Organ Culture of Fetal Small Intestine
• T Cell Response to Hen's Egg Ovomucoid(Biological Chemistry)
• Comparison of Four Distinct Idiotypes of Monoclonal Antibodies against Hen's Egg Ovomucoid(Biological Chemistry)
• A pivotal role of IL-12 in T_h 1-dependent mouse liver injury
• Interleukin-12 Augments the Generation of Autologous Tumor-reactive CD8^+ Cytotoxic T Lymphocytes from Tumor-infiltrating Lymphocytes
• Suppression of the Systemic Immune Response to Casein by Oral Administration of a Tryptic Digest of Casein
• High-pressure Proteolytic Digestion of Food Proteins : Selective Elimination of β-Lactoglobulin in Bovine Milk Whey Concentrate(Food & Nutrition)
• Molecular Cloning and Nucleotide Sequence of the Aminopeptidase T Gene of Thermus aquaticus YT-1 and Its High-level Expression in Escherichia coli(Microbiology & Fermentation Industry)
• Occurrence of High Molecular Weight EGF Complexes in Human Milk(Food & Nutrition)
• Preparation of Monoclonal Antibody against Bovine β-Lactoglobulin and Its Unique Binding Affinity(Biological Chemistry)
• Emulsifying Properties of Peptides Obtained from the Hydrolyzates of β-Casein(Food & Nutrition)
• Dietary Melibiose Regulates Th Cell Response and Enhances the Induction of Oral Tolerance
• Comparison between the Antigenicity of Native and Unfolded β-Lactoglobulin(Biological Chemistry)
• Bacteroides Induce Higher IgA Production Than Lactobacillus by Increasing Activation-Induced Cytidine Deaminase Expression in B Cells in Murine Peyer's Patches
• Development of Intestinal Flora of Human-Flora-Associated (HFA) Mice in the Intestine of their Offspring
• Mucosal Immunity in CD4-Deficient Mice : Direct Evidence for Mucosal IgA Antibody Responses
• Saccharomyces cerevisiae and Candida albicans Stimulate Cytokine Secretion from Human Neutrophil-Like HL-60 Cells Differentiated with Retinoic Acid or Dimethylsulfoxide
• Cytokine Responses of Intestinal Epithelial-Like Caco-2 Cells to Non-Pathogenic and Opportunistic Pathogenic Yeasts in the Presence of Butyric Acid
• Comparison of the Immunopotentiating Activity with Structural Characteristics among Water-soluble Polysaccharides Isolated from the Genus Bifidobacterium
• Differentiated implication of Lactobacillus GG and L. gasseri TMC0356 to immune responses of murine Peyer's patch
• PCR Method for Detecting Trace Amounts of Buckwheat (Fagopyrum spp.) in Food
• Localization of Sequential Antigenic Determinants on Bovine β-Casein with Synthetic Peptides and Antisera from Mouse, Rabbit, and Goat
• Establishment of a Primary Culture Method for Mouse Intestinal Epithelial Cells by Organ Culture of Fetal Small Intestine
• Isolation and Characterization of a Thermostable Aminopeptidase (Aminopeptidase T) from Thermus aquaticus YT-1, an Extremely Thermophilic Bacterium(Biological Chemistry)
• Preparation of Anti-bovine β-Casein Monoclonal Antibody and Analysis of the Interaction between the Antibody and β-Casein Fragment(Biological Chemistry)
• Intestinal Immune System and Prebiotics
• Food Allergy, Oral Tolerance and Immunomodulation-Their Molecular and Cellular Mechanisms
• Amelioration of D-Galactosamine-Induced Acute Liver Injury in Rats by Dietary Supplementation with Betaine Derived from Sugar Beet Molasses
• Effects of Dietary Supplementation with Betaine on a Nonalcoholic Steatohepatitis (NASH) Mouse Model
• Emulsifying Properties of a Mixture of Peptides Derived from the Enzymatic Hydrolyzates of Bovine Caseins
• Isolation and Characterization of a Prolidase from Streptococcus cremoris H61
• Purification and Properties of a Dipeptidase from Streptococcus cremoris
• Kinetic Properties of a Dipeptidase from Streptococcus cremoris
• Localization of Allergenic Reactive Sites on Hen Ovomucoid
• The Antigenic Properties of β-Lactoglobulin Examined with Mouse IgE Antibody
• Studies on the Allergenic Structure of Hen Ovomucoid by Chemical and Enzymic Fragmentation
• Reconstitution of Human Casein Micelle and Its Properties
• Properties of Glycomacropeptide and Para-K-casein Derived from Human κ-Casein and Comparison of Humanand Bovine κ-Caseins as to Susceptibility to Chymosin and Pepsin
• Molecular Weight and Conformation of HumanK-Casein and Its Interaction with Other HumanMilk Proteins

もっと見る 閉じる

スポンサーリンク