Characterization of a Water-soluble Polysaccharide Fraction with Immunopotentiating Activity from Bifidobacterium adolescentis M101-4
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概要
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The soluble and insoluble fractions obtained after sonication and centrifugation of Bifidobacterium adolescentis M101-4 cells were examined, and both of these fractions exhibited mitogenic activity in an assay of murine splenocytes and Peyer's patch cells in vitro. The soluble fraction was further treated by a 6-step procedure involving proteinase K-treatment, ultrafiltration with a 50-kDa cut-off molecular-sieving membrane, anion-exchange chromatography, dialysis, ultrafiltration through a 6-kDa cut-off membrane filter, and gel-filtration to yield a soluble high molecular weight fraction (SHF) which was effective for stimulating the proliferation of murine splenocytes. Almost three quarters of this fraction by weight was found to consist of carbohydrates containing glucose and galactose as major constituents, and the average molecular weight was estimated to be between 60,000 and 2,460,000, with the main peak at 1,550,000 Da, by the retention time of gel permeation chromatography. A structural analysis by ^1H- and ^<13>C-nuclear magnetic resonance and methylation indicated that SHF contained polysaccharides consisting of -4Galp1-, -4Glcp1-, and -6Glcp1- as the major residues, and Galf1- and -6Galf1- as the minor residues. Immunopotentiating SHF was found to contain galactofuranosyl residues as characteristic constituents which had not been previously detected in other soluble fractions from Gram-positive bacteria.
- 社団法人日本農芸化学会の論文
- 1997-02-23
著者
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Adachi Takahiro
Department Of Applied Biological Sciences School Of Agricultural Sciences Nagoya University
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Natsume Midori
Functional Food Research And Development Laboratory Meiji Seika Kaisha
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Natsume Midori
Bioscience Laboratories Meiji Seika Kaisha Ltd.
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Adachi T
Med. Res. Inst. Tokyo Med. Dent. Uni.
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HIRAYAMA Masao
Bioscience Laboratories, Meiji Seika Kaisha, Ltd.
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KAMINOGAWA Shuichi
Department of Food Science and Technology, College of Bioresource Sciences, Nihon University
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HOSONO Akira
Department of Food Science and Technology College of Bioresource Sciences, Nihon University
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Kaminogawa Shuichi
Department Of Applied Biological Chemistry Division Of Agriculture And Agricultural Life Sciences Th
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AMETANI Akio
Applied Biological Chemistry, University of Tokyo
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AMETANI Akio
Department of Applied Biological Chemistry, The University of Tokyo
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Kametani Y
Applied Biological Chemistry University Of Tokyo
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Ametani Akio
Department Of Applied Biological Chemistry The University Of Tokyo:(present Address)torrey Pines Ins
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Hosono A
Nihon Univ. Kanagawa Jpn
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Hosono Akira
Department Of Biochemistry Osaka Medical College
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Hirayama M
Meiji Seika Kaisha Ltd. Saitama Jpn
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Hirayama Masao
Bioscience Laboratories Meiji Seika Kaisha Ltd.
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ADACHI Takashi
Biosci. Labs., Meiji Seika Kansha
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LEE Jonghwa
Department of Applied Biological Chemistry, The University of Tokyo
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Lee Jonghwa
Department Of Applied Biological Chemistry The University Of Tokyo:(present Address)department Of Fo
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Kaminogawa S
Dep. Of Food Sci. And Technol. Nihon Univ.
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Kaminogawa Shuichi
Department Of Agricultural Chemistry Faculty Of Engineering The University Of Tokyo
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Lee Jonghwa
Department Of Applied Biological Chemistry The University Of Tokyo
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Hosono Akira
Department of Applied Biological Chemistry, The University of Tokyo
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