Inulo-Oligosaccharides : Continuous Production from Inulin by Immobilized Inulinase from Aspergillus niger and in vitro Utilization by Bifidobacteria

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概要

• 論文の詳細を見る

• Endoinulinase was partially purified from the culture filtrate of a filamentous fungus Aspergillus niger mutant 817. The enzyme preparation was immobilized covalently onto a porous cellulose derivative, Amino-Cellulofine. A 5% (w/v) solution (pH 5.0) of pure dahlia inulin was fed continuously into a packed-bed column reactor containing the immobilized enzyme. The operating conditions were studied to produce a mixture of oligomers with a degree of polymerization of 3 to 5 by partial hydrolysis of inulin. Inulotriose (F3) and -tetraose (F4) were purified from the hydrolysis products by preparative high-performance liquid chromatography. In vitro studies indicated that both the F3 and F4 were preferentially utilized by Bifidobacterium spp., but not by Escherichia coli or Clostridium perfringens.

• 社団法人　日本食品科学工学会の論文
• 2001-05-01

著者

• Ohta Kazuyoshi

  Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, University of Miyazaki

• 中村 太郎

  大阪市大 大学院

• 太田 一良

  Department Of Biochemistry And Applied Biosciences Faculty Of Agriculture University Of Miyazaki

• Ohta K

  Nihon Tokushu Noyaku Seizo K.k. Tokyo Jpn

• Ohta Kazuyoshi

  Department Of Agricultural Chemistry

• Ohta Kazuyoshi

  Department Of Biochemistry And Applied Biosciences Faculty Of Agriculture Miyazaki University

• NAKAMURA TOYOHIKO

  Department of Biological Resource Sciences, Faculty of Agriculture, Miyazaki University

• 中村 豊彦

  宮崎大学農学部応用生物科学科

• OGATA Yasuko

  Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, Miyazaki University

• KAMO Yoshihiro

  Bioscience Laboratories, Meiji Seika Kaisha, Ltd.

• HIRAYAMA Masao

  Bioscience Laboratories, Meiji Seika Kaisha, Ltd.

• Tanaka N

  Tokyo Inst. Technol. Kanagawa

• Ogata Yasuko

  Department Of Biochemistry And Applied Biosciences Faculty Of Agriculture Miyazaki University

• Hirayama Masao

  Bioscience Laboratories Meiji Seika Kaisha Ltd.

• Kamo Yoshihiro

  Bioscience Laboratories Meiji Seika Kaisha Ltd.

• Ohta K

  Department Of Agricultural Chemistry Faculty Of Agriculture Kyushu University

• Nakamura Toyohiko

  Department Of Biological Resource Sciences Faculty Of Agriculture Miyazaki University

• NAKAMURA Toyohiko

  Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, Miyazaki University

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