Molecular Cloning and Sequence Analysis of Two Endoinulinase Genes from Aspergillus niger
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概要
- 論文の詳細を見る
Two genomic DNAs encoding endoinulinase from Aspergillus niger 12 were cloned and sequenced. Open reading frames (ORFs) of the endoinulinase genes, inuA and inuB, both consisted of 1,548 nucleotides encoding 516 amino acids. It was suggested that the coding regions were not interrupted by introns. The ORFs differed from each other by 23 nucleotide substitutions or by eight amino acid replacements, indicating that the inuA and inuB genes arose by gene duplication. Each mature enzyme of 493 amino acids was preceded by a hydrophobic signal peptide of 23 amino acids. The enzymes contained two Cys residues and five potential sites for N-linked glycosylation. Partial amino acid sequences of the secreted enzyme suggested that it originated from the inuB gene product. The deduced amino acid sequences of the mature A. niger enzymes showed 73% identity with that of the Penicillium purpurogenum endoinulinase.
- 社団法人日本農芸化学会の論文
- 1998-09-23
著者
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Ohta Kazuyoshi
Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, University of Miyazaki
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中村 太郎
大阪市大 大学院
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太田 一良
Department Of Biochemistry And Applied Biosciences Faculty Of Agriculture University Of Miyazaki
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Ohta K
Nihon Tokushu Noyaku Seizo K.k. Tokyo Jpn
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Ohta Kazuyoshi
Department Of Agricultural Chemistry
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Ohta Kazuyoshi
Department Of Biochemistry And Applied Biosciences Faculty Of Agriculture Miyazaki University
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NAKAMURA TOYOHIKO
Department of Biological Resource Sciences, Faculty of Agriculture, Miyazaki University
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AKIMOTO Hidetoshi
Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, Miyazaki University
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中村 豊彦
宮崎大学農学部応用生物科学科
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MATSUDA Shusaku
Department of Biological Resource Sciences, Faculty of Agriculture, Miyazaki University
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TOSHIMITSU Daisuke
Department of Biological Resource Sciences, Faculty of Agriculture, Miyazaki University
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Tanaka N
Tokyo Inst. Technol. Kanagawa
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Ohta K
Department Of Agricultural Chemistry Faculty Of Agriculture Kyushu University
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Moriyama S
Department Of Biochemistry And Applied Biosciences Faculty Of Agriculture University Of Miyazaki
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Nakamura Toyohiko
Department Of Biological Resource Sciences Faculty Of Agriculture Miyazaki University
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Akimoto Hidetoshi
Department Of Biochemistry And Applied Biosciences Faculty Of Agriculture Miyazaki University
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Toshimitsu Daisuke
Department Of Biological Resource Sciences Faculty Of Agriculture Miyazaki University
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NAKAMURA Toyohiko
Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, Miyazaki University
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