Isolation and Growth Characteristics of a Nystatin-Resistant Mutant of a Thermotolerant Yeast, Hansenula polymorpha
スポンサーリンク
概要
- 論文の詳細を見る
A nystatin-resistant mutant (NR-21) of a thermotolerant yeast, Hansenula polymorpha CK-1,was isolated by mutagenesis with ethyl methanesulfonate, followed by selection for resistance to nystatin (50 units/ml). The mutant was defective in ergosterol biosynthesis. Specific growth rates (h^<-1>) of the mutant were reduced to 0.35 at 40℃ and 0.16 at 50℃ as compared with the wild type (0.53 at 40℃ and 0.28 at 50℃). The mutant grown with ergosterol-phosphatidylcholine emulsion at 50℃ incorporated ergosterol and its specific growth rate was increased to 0.41,which was comparable to that of the wild type grown under the same conditions.
- 社団法人日本生物工学会の論文
- 1987-08-25
著者
-
Ohta Kazuyoshi
Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, University of Miyazaki
-
Hayashida Shinsaku
Department of Agricultural Chemistry, Faculty of Agriculture, Kyushu University
-
Hayashida Shinsaku
Department Of Agricultural Chemistry Kyushu University:corresponding Author
-
Hayashida Shinsaku
Department Of Agricultural Chemistry
-
WIJEYARATNE Sushila
Department of Agricultural Chemistry, Kyushu University
-
Ohta K
Nihon Tokushu Noyaku Seizo K.k. Tokyo Jpn
-
Ohta Kazuyoshi
Department Of Agricultural Chemistry
-
Ohta Kazuyoshi
Department Of Agricultural Chemistry Kyushu University
-
Ohta Kazuyoshi
Department Of Biochemistry And Applied Biosciences Faculty Of Agriculture Miyazaki University
-
Wijeyaratne Sushila
Department Of Agricultural Chemistry Faculty Of Agriculture Kyushu University
-
Ohta K
Department Of Agricultural Chemistry Faculty Of Agriculture Kyushu University
-
Hayashida S
Laboratory Of Applied Microbiology Department Of Agricultural Chemistry Kyushu University
関連論文
- An extracellular endo-1,4-β-xylanase from Aspergillus japonicus : Purification, properties, and characterization of the encoding gene(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
- Cell-associated β-xylosidase from Aureobasidium pullulans ATCC 20524 : Purification, properties, and characterization of the encoding gene(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
- An Absorption Mechanism for the Decolorization of Melanoidin by Rhizoctonia sp. D-90
- Modulation of dexamethasone-induced thymocyte apoptosis by heat-shock protein 90-binding agents
- 3B10-4 糸状菌Aspergillus japonicusが生産するβ-キシロシダーゼの精製と遺伝子クローニング(酵素学・酵素工学・タンパク質工学,一般講演)
- Specific Inhibition by Cyclodextrins of Raw Starch Digestion by Fungal Glucoamylase
- A Novel Raw-Starch-Digesting Yeast α-Amylase from Lipomyces starkeyi HN-606
- The Hyperdigestion of Raw Starch by a Carbohydrate-Rich Glucoamylase from a Protease- and Glycosidase-Negative Mutant of Aspergillus awamori var. kawachi F-2035
- Molecular Cloning of the Glucoamylase I Gene of Aspergillus awamori var. kawachi for Localization of the Raw-starch-affinity Site(Microbiology & Fermentation Industry)
- Evidence for Post-translational Generation of Multiple Forms of Aspergillus awamori var. kawachi Glucoamylase(Biological Chemistry)
- Linear and Circular Conformation of Pock-Forming Plasmid pSAl of Thiostrepton-Producing Streptomyces azureus ATCC 14921
- Transfection and Transformation Systems for Pock-Forming and Thiostrepton-Producing Streptomyces azureus
- A Novel Type of Life Cycle "Delayed Homothallism" in Saccharomyces cerevisiae wy2 Showed Slow Interconversion of Mating-type
- A Novel Method for Breeding Polyploid Cells by Heat-induced Endomitotic Diploidization in Saccharomyces cerevisiae
- Frequent Occurrence of Diploid a or α Sporogenous Mater Cells and Construction of Ploidy Series in the Sake Yeast Kyokai No. 9
- Growth Promotion of Fluorescent Pseudomonads and Control of Potato Common Scab in Field Soil with Non-antibiotic Actinomycete-biofertilizer
- Isolation of Anti-Algal Pseudomonas stutzeri Strains and Their Lethal Activity for Chattonella antiqua(Microbiology & Fermentation Industry)
- Role of the Carbohydrate Moiety of a Glucoamylase from Aspergillus awamori var.kawachi in the Digestion of Raw Starch
- The Mechanism of Binding of Glucoamylase I from Aspergillus awamori var. kawachi to Cyclodextrins and Raw Starch
- Control of Potato Common Scab with an Antibiotic Biofertilizer Produced from Swine Feces Containing Streptomyces albidoflavm CH-33
- Lipid Composition of a Thermotolerant Yeast, Hansenula polymorpha
- Antitumor Activity of an Alkali Extract of Clostridium saccharoperbutylacetonicum Cells
- Evidence for Raw Starch-affinity Site on Aspergillus awamori Glucoamylase I
- Production and Purification of a Novel Type of CMCase from Humicola grisea var. thermoidea YH-78
- Raw Starch-digestive Chitin-immobilized Amylase from a Protease-Glycosidase-less Mutant of Aspergillus awamori var. kawachi
- Enzymatic Decolorization of Melanoidin by Coriolus sp. No. 20
- High Concentration-Ethanol Fermentation of Raw Ground Corn
- Purification and Properties of Thermostable Xylanase from Talaromyces byssochlamydoides YH-50
- Raw Starch-digestive Glucoamylase Productivity of Protease-less Mutant from Aspergillus awamori var. kawachi
- Promotive and Inhibitory Effects of Raw Starch Adsorbable Fragments from Pancreatic α-Amylase on Enzymatic Digestions of Raw Starch(Biological Chemistry)
- Multiplicity and Preferential Inactivation by Proteolysis as to Raw Starch-digestibility of Bacterial α-Amylases(Microbiology & Fermentation Industry)
- Reproductive parameters of the chub mackerel Scomber japonicus estimated from human chorionic gonadotropin-induced final oocyte maturation and ovulation in captivity
- Production of Potato Common Scab-antagonistic Biofertilizer from Swine Feces with Streptomyces albidoflavus(Microbiology & Fermentation Industry)
- Conversion of Geotrichum candidum Endocellulase I to Endocellulase II by Limited Proteolysis(Biological Chemistry)
- Production and Characteristics of Two Types of Endocellulase from Geotrichum candidum(Biological Chemistry)
- Temperature-Sensitive Mutants of a Thermotolerant Yeast, Hansenula polymorpha
- Isolation and Growth Characteristics of a Nystatin-Resistant Mutant of a Thermotolerant Yeast, Hansenula polymorpha
- 315 Lipid Composition of a Thermotolerant Yeast Hansenula polymorpha
- Structure of a New Rhamnose Polysaccharide, Clostrhamnan, Isolated from Antitumor Hot Water Extract of Clostridium saccharoperbutylacetonicum Cells(Biological Chemistry)
- Functional Characterization and Evolutionary Implication of the Internal 157-Amino-Acid Sequence of an Exoinulinase from Penicillium sp. Strain TN-88(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
- Occurrence of Two Forms of Extracellular Endoinulinase from Aspergillus niger Mutant 817
- Quantitative Expression Analysis of Inulinase Gene Cluster of Penicillium sp. Strain TN-88(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
- Acidophilic Xylanase from Aureobasidium pullulans : Efficient Expression and Secretion in Pichia pastoris and Mutational Analysis(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
- 糸状菌イヌリナーゼ : 酵素化学, 分子生物学およびバイオテクノロジー
- Molecular Cloning and Characterization of an Exoinulinase Gene from Aspergillus niger Strain 12 and Its Expression in Pichia pastoris
- Purification and Properties of an Extracellular Exoinulinase from Penicillium sp. Strain TN-88 and Sequence Analysis of the Encoding Gene(Biochemistry & Molecular Biology)
- Aspergillus niger No.12株の細胞内エキソおよびエンド型イヌリナーゼの精製と性質
- Purification and Characterization of an Acidophilic Xylanase from Aureobasidium pullulans var.melanigenum and Sequence Analysis of the Encoding Gene
- Inulo-Oligosaccharides : Continuous Production from Inulin by Immobilized Inulinase from Aspergillus niger and in vitro Utilization by Bifidobacteria
- Molecular Cloning and Sequence Analysis of an Endoinulinase Gene from Penicillium sp. Strain TN-88
- 618 糸状菌Penicillium sp. TN-88株由来エキソ型イヌリナーゼ遺伝子のクローニングと解析
- Transcriptional Analysis of Two Endoinulinase Genes inuA and inuB in Aspergillus niger and Nucleotide Sequences of Their Promoter Regions
- Molecular Cloning and Sequence Analysis of Two Endoinulinase Genes from Aspergillus niger
- Production, Purification and Properties of an Endoinilinase of Penicillium sp. TN-88 That Liberates Inulotriose
- Aspergillus nigerの生産する細胞外エンド型イヌリナ-ゼ(P-3)の固定化と性質
- Ethanol Production from Jerusalem Artichoke Tubers by Aspergillus niger and Saccharomyces cerevisiae
- 野生株Aspergillus niger No. 12およびその変異株No. 817の生産する細胞外エンド型イヌリナーゼ : 酵素
- Continuous Production of Fructose Syrups from Inulin by Immobilized Inulinase from Aspergillus niger Mutant 817
- 固定化イヌリナーゼによるイヌリンからの高フルクトース・シロップの連続生産 : 酵素
- Proline-specific Endopeptidase from Flavobacterium meningosepticum: Physicochemical Properties
- Purification and Properties of an Extracellular β-Xylosidase from Aspergillus japonicus and Sequence Analysis of the Encoding Gene
- Purification and Properties of Family-10 Endo-1,4-β-Xylanase from Penicillium citrinum and Structural Organization of Encoding Gene(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
- Purification and Properties of an Extracellular Endo-1, 4-β-Xylanase from Penicillium citrinum and Characterization of the Encoding Gene(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
- Purification and Properties of an Extracellular Inulinase from Rhizopus sp. Strain TN-96(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
- An Early Warning System for Illegal Access Based on Distributed Network Monitoring, Vol.J86-B,No.3, pp.410-418
- Mobility Management for IP/LEO Satellite Networks Using Geographical Location
- A New Scheme of Combining Advanced Packet Discard and Dynamic Bandwidth Allocation for Low Delay/Low litter Realtime Communication Using CBQ/ALTQ(Special Issue on Network Software)
- Proposal of Dynamic Bandwidth Allocation Technique for Low Delay/Low Jitter Realtime Communication and Its Evaluation by Using CBQ (Special Issue on New Developments on QoS Technologies for Information Networks)
- A Real-Time Intrusion Detection System (IDS) for Large Scale Networks and Its Evaluations (Special Issue on New Paradigms in Network Management)
- A Proposal of Event Correlation for Distributed Network Fault Management and Its Evaluation (Special Issue on Distributed Processing for Controlling Telecommunications Systems)
- The Dynamic Symptom Isolation Algorithm for Network Fault Management and Its Evaluation (Special Issue on the Latest Development of Telecommunication Research)
- Construction and Utilization Experiment of Multimedia Education System Using Satellite ETS-V and Internet (Special Issue on Educational System using Multimedia and Communication Technology)
- Problems in Management Information Retrieval for High-Speed Networks and a Proposed Solution (Special Issue on Multimedia on Demand)
- MOLECULAR CLONING OF ESTROGEN AND ANDROGEN RECEPTORS AND THEIR EXPRESSION PROFILE DURING SEX CHANGE IN THE PROTOGYNOUS WRASSE, PSEUDOLABRUS SIEBOLDI
- Steroidogenesis in Ovarian Follicles of Chub Mackerel, Scomber japonicus(Reproductive Biology)
- MOLECULAR CLONING AND CHARACTERIZATION OF DMRT GENES FROM THE PUFFERFISH TAKIFUGU RUBRIPES(Cell Biology and Morphology & Developmental Biology,Abstracts of papers presented at the 74^ Annual Meeting of the Zoological Society of Japan)
- STEROIDOGENIC PATHWAYS IN THE VITELLOGENIC OVARIAN FOLLICLES OF THE CHUB MACKEREL, SCOMBER JAPONICUS, AND KYUSEN WRASSE, HALICHOERES POECILOPTERUS : DIFFERENT MODES OF ESTROGEN SYNTHESIS IN GONOCHORISTIC AND PROTOGYNOUS TELEOSTS
- DIURNAL RHYTHM OF GAMETOGENESIS AND SERUM SEX STEROIDS IN FEMALE AND MALE PSEUDOLABRUS SIEBOLDI, A PROTOGYNOUS WRASSE
- Steroidogenic Pathway to Estradiol-17β Synthesis in the Ovarian Follicles of the Protogynous Wrasse, Pseudolabrus sieboldi(Reproductive Biology)
- Environmental Effents on Ethanol Tolerance of Zymomonas mobilis : Ethanol Tolerance of Zymomonas mobilis (I)
- Preparation and Enzymatic Properties of Aspergillus niger Endoinulinase Immobilized onto Various Polysaccharide Supports
- Expression of AMP-activated Protein Kinase Subunit Isoforms in Masseter and Tibialis Anterior Muscles of Mice before and after Weaning
- Characteristics of Raw-Starch-Digesting Glucoamylase from Thermophilic Rhizomucor pusillus
- Enzymatic Modification of Glucoamylase of Aspergillus awamori var. kawachi
- An Avicel-affinity Site in an Avicel-digesting Exocellulase from a Trichoderma viride Mutant
- Hypoxia induces expression and activation of AMPK in rat dental pulp cells
- 188 TWO CASES OF ANCA-ASSOCIATED CRESCENTIC GLOMERULONEPHRITIS INDUCED BY PROPYLTHIOURACIL
- Disruption of ptsG gene and manXYZ operon of ethanol-producing Escherichia coli KO11 : Effects on glucose and xylose utilization and ethanol production(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- Ethanol production from non-pretreated napiergrass through a simultaneous saccharification and fermentation process followed by a pentose fermentation with Escherichia coli KO11(BIOCHEMICAL ENGINEERING)
- Disruption of ptsG gene and manXYZ operon of ethanol-producing Escherichia coli KO11 : Effects on glucose and xylose utilization and ethanol production
- Aspergillus nigerの生産する細胞外エキソ型イヌリナ-ゼ(P-I)の性質について
- Aspergillus kawachiiの脂質代謝関連酵素に関する検討
- Relationship between Carbohydrate Moiety and Thermostability of β-Glucosidase from Mucor miehei YH-10
- Characterization of a glycoside hydrolase family-51 α-L-arabinofuranosidase gene from Aureobasidium pullulans ATCC 20524 and its encoded product(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
- Production and Characterization of Thermostable Xylanase from Talaromyces byssochlamydoides YH-50