Production, Purification and Properties of an Endoinilinase of Penicillium sp. TN-88 That Liberates Inulotriose
スポンサーリンク
概要
- 論文の詳細を見る
In a screening of inulinolytic fungi, Penicillium sp. TN-88 was selected as the best producer of inulinase. This strain showed a high inulinase productivity of 9.9 U/ml with a ratio od inulinase activity to invertase activity (I/S ratio)of 11.2 in the culture filtrate when grown in a liquid medium containing inulinas the carbon source at 30℃ for 4 d. An endoinulinase, P-II, was 45-fold purified from the culture filtrate by DEAE-Cellulofine A-500 and Q-Sepharose HP chromatographies. The enzyme was homogeneous, as judged by SDS-polyacrylaminde gel electrophoresis, with an apparent _r of 68.0 kDa. The specific activity was 105 U/mg. The enzyme activity was highest at pH 5.2 and 50℃. Endoinulinase P-II hydrolyzed inulin to the extent of 70% and liberated inulotriose as the main product, but lacked activity toward sucrose, raffinose or levan. The enzyme activity was completely inactivated by Ag^+, Hg^<2+> or p-chloromercuribenzoate and by chemical modification with carbodiimide or N-bromosuccinimide. The apparent K_m value for inulin was 0.20 mM at 40℃ and pH 5.0. The N-terminal amino acid sequence of the enzyme was 1-DDYRPAFFHFC PAENXMNEPN GLIQIXSTXH-30.
- 社団法人日本生物工学会の論文
- 1997-10-25
著者
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Ohta Kazuyoshi
Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, University of Miyazaki
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中村 太郎
大阪市大 大学院
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SUIKO Masahito
Department of Biochemistry and Applied Biosciences, University of Miyazaki
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太田 一良
Department Of Biochemistry And Applied Biosciences Faculty Of Agriculture University Of Miyazaki
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Ohta K
Nihon Tokushu Noyaku Seizo K.k. Tokyo Jpn
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Ohta Kazuyoshi
Department Of Agricultural Chemistry
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Ohta Kazuyoshi
Department Of Biochemistry And Applied Biosciences Faculty Of Agriculture Miyazaki University
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NAKAMURA TOYOHIKO
Department of Biological Resource Sciences, Faculty of Agriculture, Miyazaki University
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中村 豊彦
宮崎大学農学部応用生物科学科
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MATSUDA Shusaku
Department of Biological Resource Sciences, Faculty of Agriculture, Miyazaki University
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SHITARA AKICHIKA
Department of Biological Resource Sciences, Faculty of Agriculture, Miyazaki University
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MATSUO TADAHIKO
Department of Biological Resource Sciences, Faculty of Agriculture, Miyazaki University
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Tanaka N
Tokyo Inst. Technol. Kanagawa
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Suiko M
Miyazaki Univ. Miyazaki Jpn
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Ohta K
Department Of Agricultural Chemistry Faculty Of Agriculture Kyushu University
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Moriyama S
Department Of Biochemistry And Applied Biosciences Faculty Of Agriculture University Of Miyazaki
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Matsuo Tadahiko
Department Of Biological Resource Sciences Faculty Of Agriculture Miyazaki University
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Nakamura Toyohiko
Department Of Biological Resource Sciences Faculty Of Agriculture Miyazaki University
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Shitara Akichika
Department Of Biological Resource Sciences Faculty Of Agriculture Miyazaki University
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Suiko Masahito
Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, University of Miyazaki
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NAKAMURA Toyohiko
Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, Miyazaki University
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SUIKO Masahito
Department of Agricultural Chemistry, Miyazaki University
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