An extracellular endo-1,4-β-xylanase from Aspergillus japonicus : Purification, properties, and characterization of the encoding gene(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
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概要
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An extracellular endo-1,4-β-xylanase with specific activity of 566 U/mg was purified from the culture filtrate of a filamentous fungus, Aspergillus japonicus strain MU-2, grown on oat spelt xylan. The purified enzyme showed a single band on SDS-PAGE with an apparent M_r of 25.1kDa. Xylanase activity was optimal at pH 5.0 and 60℃. The xylanase gene (xynA) encoded a 42 residue prepropeptide and a 191 residue mature protein. The XynA protein showed the highest sequence identity of 69% to Aspergillus niger XynB (DQ174549). which belongs to the glycoside hydrolase family 11.
著者
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Ohta Kazuyoshi
Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, University of Miyazaki
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Wakiyama Motoki
Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, University of Miyazaki
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Yoshihara Koji
Department of Applied Chemistry, Faculty of Engineering, University of Miyazaki
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Hayashi Sachio
Department of Applied Chemistry, Faculty of Engineering, University of Miyazaki
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Yoshihara Koji
Department Of Applied Chemistry Faculty Of Engineering University Of Miyazaki
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Hayashi Sachio
Department Of Applied Chemistry Faculty Of Engineering University Of Miyazaki
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Wakiyama Motoki
Department Of Biochemistry And Applied Biosciences Faculty Of Agriculture University Of Miyazaki
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Ohta Kazuyoshi
Department Of Agricultural Chemistry
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