Maltotriose-producing Amylase from Microbacterium imperiale(Microbiology & Fermentation Industry)

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概要

• 論文の詳細を見る

• A maltotriose-producing amylase, which hydrolyzes the α-1,4-glucosidic linkages in starch with maltotriose units was found in the culture filtrate of a strain of the genus Microbacterium newly isolated from soil. The enzyme was purified almost to homogeneity by ammonium sulfate fractionation, DEAE-Sepharose column chromatography and Sephadex G-150 column chromatography. Its optimum pH and temperature were around 6.5-7.0 and around 50℃, respectively. The enzyme was protected from heat in the presence of calcium ion. Its molecular weight was estimated to be about 40,000 by the gel filtration method. Typical sugar composition of hydrolysis products from liquefied starch was about 55-60% of maltotriose, 10-20% of maltose, 1-3% of glucose and 20-40% of other higher oligosaccharides.

• 社団法人日本農芸化学会の論文
• 1991-03-23

著者

• Hayashi Sachio

  Department of Applied Chemistry, Faculty of Engineering, University of Miyazaki

• Hayashi Sachio

  Department Of Applied Chemistry Faculty Of Engineering University Of Miyazaki

• Hayashi Sachio

  Department Of Materials Science Faculty Of Engineering Miyazaki University

• Kitajima Mariko

  Department Of Industrial Chemistry Faculty Of Engineering Miyazaki University

• Takasaki Yoshiyuki

  Department of Materials Science, Faculty of Engineering, Miyazaki University

• 今田 清久

  Miyazaki Univ.

• 今田 清久

  宮崎大・工・物質工

• Imada Kiyohisa

  Department Of Applied Chemistry Faculty Of Engineering Miyazaki University

• Hayashi S

  Miyazaki Univ.

• Hayashi Satio

  Department Of Materials Science Faculty Of Engineering Miyazaki University

• IMADA Kiohisa

  Department of Industrial Chemistry, Faculty of Engineering, Miyazaki University

• TSURUTA Takuro

  Department of Industrial Chemistry, Faculty of Engineering, Miyazaki University

• NONOGUCHI Masaharu

  Department of Industrial Chemistry, Faculty of Engineering, Miyazaki University

• Takasaki Yoshiyuki

  Department Of Applied Chemistry Faculty Of Engineering Miyazaki University

• Takasaki Yoshiyuki

  Laboratory Of Biophysical Chemistry College Of Agriculture Osaka Prefecture University:dainippon Phe

• Tsuruta Takuro

  Department Of Industrial Chemistry Faculty Of Engineering Miyazaki University

• Nonoguchi Masaharu

  Department Of Industrial Chemistry Faculty Of Engineering Miyazaki University

• Kitajima Mariko

  Department of Biofunctional Molecular Chemistry, Graduate School of Pharmaceutical Sciences, Chiba University

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