Preparation and Enzymatic Properties of Aspergillus niger Endoinulinase Immobilized onto Various Polysaccharide Supports
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概要
- 論文の詳細を見る
An extracellular endoinulinase P-III from Aspergillus niger 12 has been immobilized covalently onto bromoacetyl-cellulose (BAC), cellulose-carbonate (CC) and CNBr-activated Sepharose 4B (CAS) with the activity yields of 11.9, 24.1 and 19.3%, respectively, under optimal reaction conditions for the immobilization. All three immobilized enzymes increased the resistance to inactivation by p-chloromercuribenzoate or Fe3+. Mn2+ stimulated the activities of the immobilized endoinulinases onto CC (160%) and CAS (168%) more remarkably than those of free and BAC-immobilized enzymes (124%). The marked rise from 45 to 60°C in the optimal temperature for inulin hydrolysis was observed in the CAS-immobilized enzyme. The pH stability decreased on the acidic side and increased slightly on the alkaline side in the BAC-immobilized enzyme, and shifted to the acidic side in the CC-immobilized enzyme. The Km value decreased in the BAC-immobilized enzyme and increased in the CAS-immobilized enzyme.
- 社団法人 日本食品科学工学会の論文
- 2000-02-01
著者
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Ohta Kazuyoshi
Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, University of Miyazaki
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Ohta Kazuyoshi
Department Of Agricultural Chemistry
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Ohta Kazuyoshi
Department Of Biological Resource Sciences Faculty Of Agriculture Miyazaki University
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NAKAMURA TOYOHIKO
Department of Biological Resource Sciences, Faculty of Agriculture, Miyazaki University
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ZAITA Norihiro
Department of Biological Resource Sciences, Faculty of Agriculture, Miyazaki University
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FUKUSHIGE Tomoaki
Department of Biological Resource Sciences, Faculty of Agriculture, Miyazaki University
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TOKUDA Masaki
Department of Biological Resource Sciences, Faculty of Agriculture, Miyazaki University
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Zaita Norihiro
Department Of Biological Resource Sciences Faculty Of Agriculture Miyazaki University
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Tokuda Masaki
Department Of Biological Resource Sciences Faculty Of Agriculture Miyazaki University
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Nakamura Toyohiko
Department Of Biological Resource Sciences Faculty Of Agriculture Miyazaki University
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Fukushige Tomoaki
Department Of Biological Resource Sciences Faculty Of Agriculture Miyazaki University
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NAKAMURA Toyohiko
Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, Miyazaki University
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