Ethanol Sulfation by the Human Cytosolic Sulfotransferases: A Systematic Analysis

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概要

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• Ethyl sulfate, a minor and direct ethanol metabolite in adult human body, has been implicated as a biomarker for alcohol consumption and in utero exposure to ethanol. To understand better the physiological relevance of the sulfation of ethanol, it is important to clarify the cytosolic sulfotransferase (SULT) enzymes that are responsible for ethanol sulfation. The present study aimed to identify the major ethanol-sulfating human SULTs and to investigate the sulfation of ethanol under the metabolic setting. A systematic analysis revealed four ethanol-sulfating SULTs, SULT1A1, SULT1A2, SULT1A3, and SULT1C4, among the eleven human SULT enzymes previously prepared and purified. A metabolic labeling study demonstrated the generation and release of ethyl [35S]sulfate in a concentration-dependent manner by HepG2 human hepatoma cells labeled with [35S]sulfate in the presence of different concentrations of ethanol. Cytosol or S9 fractions of human lung, liver, and small intestine were examined to verify the presence of ethanol-sulfating activity in vivo. Of the three human organs, the small intestine displayed the highest activity.

著者

• SUIKO Masahito

  Department of Biochemistry and Applied Biosciences, University of Miyazaki

• Liu Ming-Yih

  National Synchrotron Radiation Research Center, Taiwan

• Sakakibara Yoichi

  Department Of Biochemistry And Applied Biosciences Faculty Of Agriculture University Of Miyazaki

• Kurogi Katsuhisa

  Department Of Biochemistry And Applied Biosciences University Of Miyazaki

• Liu Ming-cheh

  Department Of Pharmacology College Of Pharmacy The University Of Toledo

• Kurogi Katsuhisa

  Department of Pharmacology, College of Pharmacy and Pharmaceutical Sciences, The University of Toledo

• Davidson Garrett

  Department of Pharmacology, College of Pharmacy and Pharmaceutical Sciences, The University of Toledo

• Mohammed Yasir

  Department of Pharmacology, College of Pharmacy and Pharmaceutical Sciences, The University of Toledo

• Williams Frederick

  Department of Pharmacology, College of Pharmacy and Pharmaceutical Sciences, The University of Toledo

• Liu Ming-Cheh

  Department of Pharmacology, College of Pharmacy and Pharmaceutical Sciences, The University of Toledo

• Suiko Masahito

  Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, University of Miyazaki

• LIU Ming-Cheh

  Department of Biochemistry, The University of Texas Health Center at Tyler

• Liu Ming-Yih

  National Synchrotron Radiation Research Center

• SUIKO Masahito

  Department of Agricultural Chemistry, Miyazaki University

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