Preparation of 3'-Phosphoadenosine 5'-Phospho^<35>S-sulfate Using ATP Sulfurylase and APS Kinase from Bacillus stearothermophilus: Enzymatic Synthesis and Purification
スポンサーリンク
概要
- 論文の詳細を見る
Radioactive PAPS was synthesized with a predetermined specific activity using enzymes purified from Bacillus stearothermophilus. The reaction system, incorporating 1.5 mCi of ^<35>S-H_2SO_4 and 500 nmol of ATP, produced 582μCi of ^<35>S-PAPS. The synthesized ^<35>S-PAPS was purified by ECTEOLA cellulose chromatography, followed by desalting with DOWEX 50W-X8. The final yield was 206μmol of ^<35>S-PAPS with a radioactivity of 411μCi.
- 社団法人日本農芸化学会の論文
- 1993-11-23
著者
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榊原 陽一
宮崎大学農学部応用生物科学科
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Sakakibara Yoichi
Interdisciplinary Graduate School Of Agriculture And Engineering Univ. Of Miyazaki
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SAKAKIBARA Yoichi
Department of Biochemistry and Applied Biosciences, University of Miyazaki
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LIU Ming-Cheh
Department of Pharmacology, College of Pharmacy, The University of Toledo
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SUIKO Masahito
Department of Biochemistry and Applied Biosciences, University of Miyazaki
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Nakajima Hiroo
Tsujigiwa Daigo Clinic 1-19 Daigo Shinmachi-uramachi Fushimi-ku Kyoto 601-1326 Jpn
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Liu M‐c
Dep. Of Pharmacology Coll. Of Pharmacy The Univ. Of Toledo
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Sakakibara Yoichi
Department Of Biochemistry And Applied Biosciences Faculty Of Agriculture University Of Miyazaki
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Nakajima H
Division Of Dental Biomaterials Science Department Of Restorative And Biomaterials Sciences Meikai U
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Saeki Y
Department Of Biochemistry The University Of Texas Health Center
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Fernando Peter
Department of Biological Resource Sciences, Miyazaki University
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Karakawa Atsushi
Department of Biological Resource Sciences, Miyazaki University
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Ibuki Hiroshi
Unitika Research and Development Center
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Nakajima Hiroshi
Unitika Research and Development Center
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Sakakibara Yoichi
Department Of Forest Science And Resources College Of Bioresource Sciences Nihon University
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Karakawa Atsushi
Department Of Biological Resource Sciences Miyazaki University
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Liu Ming-cheh
Department Of Pharmacology College Of Pharmacy The University Of Toledo
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Suiko Masahito
Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, University of Miyazaki
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LIU Ming-Cheh
Department of Biochemistry, The University of Texas Health Center at Tyler
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SUIKO Masahito
Department of Agricultural Chemistry, Miyazaki University
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