In vivo Visualization of the Distribution of a Secretory Protein in Aspergillus oryzae Hyphae Using the RntA-EGFP Fusion Protein(Microbiology & Fermentation Technology)

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概要

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• A fusion gene encoding ribonuclease T_1-EGFP (rntA-egfp) was constructed and expressed to use it as a tool for studies on the secretory pathway in Aspergillus oryzae. The successful secretion of the intact RntA-EGFP fusion protein was detected by fluorescence measurement and Western analysis. With use of the RntA-EGFP system, we were able to see high fluorescence at hyphal tips and observe concentrated fluorescence at septa in basal cells during growth at optimal conditions. Cold or heat shock during growth caused the accumulation of EGFP fluorescence in vacuoles.

• 社団法人日本農芸化学会の論文
• 2003-02-23

著者

• Kitamoto Katsuhiko

  Department of Biotechnology, The University of Tokyo

• Nakajima Hiroo

  京都府立医科大学 内分泌･乳腺外科学部門

• Nakajima Hiroo

  Tsujigiwa Daigo Clinic 1-19 Daigo Shinmachi-uramachi Fushimi-ku Kyoto 601-1326 Jpn

• Kitamoto K

  Univ. Tokyo Tokyo Jpn

• Kitamoto K

  National Research Institute Of Brewing

• Kitamoto Katsuhiko

  Dep. Of Biotechnology Graduate School Of Agricultural And Life Sciences The Univ. Of Tokyo

• MARUYAMA Jun-ichi

  Department of Biotechnology, The University of Tokyo

• Maruyama J

  Dep. Of Biotechnology Graduate School Of Agricultural And Life Sciences The Univ. Of Tokyo

• NAKAJIMA Harushi

  Department of Biotechnology, The University of Tokyo

• MASAI Kumiko

  Department of Biotechnology, University of Tokyo

• Kitamoto Katsuhiko

  Department Of Biotechnology University Of Tokyo

• Maruyama Jun-ichi

  Dep. Of Biotechnology Graduate School Of Agricultural And Life Sciences The Univ. Of Tokyo

• Kitamoto Katsuhiko

  National Research Institute Of Brewing:(present Address)department Of Biotechnology The University O

• Nakajima H

  Department Of Endocrine Breast Surgery Kyoto Prefectural University Of Medicine

• Masai Kumiko

  Department Of Biotechnology University Of Tokyo

• Kitamoto Katsuhiko

  Department Of Applied Biological Chemistry And Department Of Applied Biological Engineering Graduate

• Maruyama Jun-ichi

  Department Of Biotechnology Graduate School Of Agricultural And Life Sciences The University Of Toky

• Nakajima Harushi

  Department Of Agricultural Chemistry The University Of Tokyo

• Nakajima Hidenori

  Exploratory Research Laboratories Fujisawa Pharmaceutical Co. Ltd

• Kimura K

  Department Of Biomedical Sciences Graduate School Of Veterinary Medicine Hokkaido University

• Maruyama Junichi

  Department Of Biotechnology The University Of Tokyo

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