Cloning and Characterization of the cpyA Gene Encoding Intracellular Carboxypeptidase from Aspergillus nidulans
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概要
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Carboxypeptidase Y (CPY) has been used as a maker enzyme for investigations on intracellular transport of vacuolar proteins and on vacuolar biogenesis in Saccharomyces cerevisiae. We describe the cloning and characterization of the CPY homologue encoding gene (cpyA) from the filamentous fungus Aspergillus nidulans. The cpyA gene has one intron and encodes a protein with 552 amino acids containing a putative signal sequence and pro-sequence. The predicted CpyA protein is highly similar in sequence with carboxypeptidases from several yeast species and contains a catalytic triad (Asp-His-Ser) like that of serine carboxypeptidase. The cpyA disruptant cells showed reduced levels of intracellular carboxypeptidase. These results suggest that the cpyA gene encodes a vacuolar carboxypeptidase in A. nidulans.
- 社団法人日本農芸化学会の論文
- 2001-05-23
著者
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Kitamoto Katsuhiko
Department of Biotechnology, The University of Tokyo
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NAKAJIMA Harushi
Department of Biotechnology, The University of Tokyo
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Kitamoto Katsuhiko
Department Of Applied Biological Chemistry And Department Of Applied Biological Engineering Graduate
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OHSUMI Keisuke
Department of Biotechnology, The University of Tokyo
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MATSUDA Yutaka
Department of Biotechnology, The University of Tokyo
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Ohsumi Keisuke
Department Of Biotechnology The University Of Tokyo
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Nakajima Harushi
Department Of Agricultural Chemistry The University Of Tokyo
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Matsuda Yutaka
Department Of Biotechnology The University Of Tokyo
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