Identification and Characterization of Scpl5, a Protein from Streptomyces coelicolor A3(2) Inducing Neurites in PC12 Cells(Biochemistry & Molecular Biology)

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概要

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• We previously showed that a fungal protein, p15, induces neurite outgrowth and differentiation of rat pheochromocytoma PC12 cells. We report here the identification and characterization of a protein similar to p15, found in Streptomyces coelicolor A3(2). This hypothetical protein, tentatively named Scpl5, has significant similarity with p15, including conserved positions of four cysteine residues involved in the formation of essential disulfide bonds in p15. Hexahistidine-tagged recombinant Scp15 proteins were produced in Es-cherichia coli, purified, and analyzed for their neuriteinducing activity. Although they were less active than p15, they dose-dependently induced neurites and the expression of neurofilament M. Neurite outgrowth by Scp15 was inhibited by nicardipine, suggesting that Scp15 induces neurites via activation of a calcium signaling pathway.

• 社団法人日本農芸化学会の論文
• 2003-01-23

著者

• Kitamoto Katsuhiko

  Department of Biotechnology, The University of Tokyo

• Arioka Manabu

  Department Of Biotechnology University Of Tokyo

• Arioka M

  Department Of Biotechnology The University Of Tokyo

• Arioka Manabu

  東京大学

• Arioka Manabu

  Department Of Biotechnology The University Of Tokyo

• Kitamoto K

  Univ. Tokyo Tokyo Jpn

• Kitamoto K

  National Research Institute Of Brewing

• Kitamoto Katsuhiko

  Dep. Of Biotechnology Graduate School Of Agricultural And Life Sciences The Univ. Of Tokyo

• NAKASHIMA Satoru

  Department of Biotechnology, University of Tokyo

• WAKATSUKI Shuji

  Department of Biotechnology, University of Tokyo

• YOKOYAMA Tatsuya

  Department of Biotechnology, University of Tokyo

• Wakatsuki Shuji

  Department Of Biotechnology University Of Tokyo:(present Address)department Of Growth Regulation Ins

• Kitamoto Katsuhiko

  Department Of Biotechnology University Of Tokyo

• Yokoyama Tatsuya

  Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni

• Yokoyama Tatsuya

  Department Of Biotechnology University Of Tokyo

• Kitamoto Katsuhiko

  National Research Institute Of Brewing:(present Address)department Of Biotechnology The University O

• Kitamoto Katsuhiko

  Department Of Applied Biological Chemistry And Department Of Applied Biological Engineering Graduate

• Nakashima Sadahiko

  Department Of Biotechnology University Of Tokyo

• Kimura K

  Department Of Biomedical Sciences Graduate School Of Veterinary Medicine Hokkaido University

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