Development of a Modified Positive Selection Medium That Allows to Isolate Aspergillus oryzae Strains Cured of the Integrated niaD-Based Plasmid
スポンサーリンク
概要
- 論文の詳細を見る
The nitrate reductase gene (niaD) is the most frequently utilized as a selectable marker for homologous integration at the niaD locus of Aspergillus oryzae. In this study we developed a method for curing of the niaD-based plasmid integrated on the A. oryzae genome. Positive selection using a modified chlorate medium containing leucine as a nitrogen source enabled efficient isolation of the strains deficient in nitrate assimilation from the niaD+ transformant. PCR analysis of the strains confirmed that the homologously integrated plasmid carrying the h2b-egfp fusion gene was cured by intrachromosomal recombination which was accompanied by the loss of the EGFP-fluorescence.
- 社団法人 日本農芸化学会の論文
- 2005-12-23
著者
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Kitamoto Katsuhiko
Department of Biotechnology, The University of Tokyo
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Kitamoto K
Univ. Tokyo Tokyo Jpn
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Kitamoto K
National Research Institute Of Brewing
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Kitamoto Katsuhiko
Dep. Of Biotechnology Graduate School Of Agricultural And Life Sciences The Univ. Of Tokyo
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MARUYAMA Jun-ichi
Department of Biotechnology, The University of Tokyo
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JUVVADI Praveen
Department of Biotechnology, The University of Tokyo
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Maruyama J
Dep. Of Biotechnology Graduate School Of Agricultural And Life Sciences The Univ. Of Tokyo
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JUVVADI Praveen
Dept. Biotech., Univ. Tokyo
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Watanabe Taisuke
RIKEN (The Institute of Physical and Chemical Research)
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ISHI Kazutomo
Department of Biotechnology, The University of Tokyo
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WATANABE Taisuke
Department of Biotechnology, The University of Tokyo
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Ishi Kazutomo
Dept. Biotechnol., Univ. Tokyo
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Kitamoto Katsuhiko
Department Of Biotechnology University Of Tokyo
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Maruyama Jun-ichi
Dep. Of Biotechnology Graduate School Of Agricultural And Life Sciences The Univ. Of Tokyo
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Juvvadi Praveen
Dept. Biotech. Univ. Tokyo
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Kitamoto Katsuhiko
National Research Institute Of Brewing:(present Address)department Of Biotechnology The University O
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Ishi Kazutomo
Department Of Biotechnology The University Of Tokyo
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Kitamoto Katsuhiko
Department Of Applied Biological Chemistry And Department Of Applied Biological Engineering Graduate
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Juvvadi Praveen
Department Of Biotechnology The University Of Tokyo
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Maruyama Jun-ichi
Department Of Biotechnology Graduate School Of Agricultural And Life Sciences The University Of Toky
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Kimura K
Department Of Biomedical Sciences Graduate School Of Veterinary Medicine Hokkaido University
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Maruyama Junichi
Department Of Biotechnology The University Of Tokyo
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Watanabe Taisuke
Department of Bioscience and Biotechnology, Faculty of Agriculture, University of the Ryukyus
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