Cloning and Overexpression of β-N-Acetylglucosaminidase Encoding Gene nagA from Aspergillus oryzae and Enzyme-catalyzed Synthesis of Human Milk Oligosaccharide(Microbiology & Fermentation Technology)
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概要
- 論文の詳細を見る
We isolated a β-N-acetylglucosaminidase encoding gene from the filamentous fungus Aspergillus oryzae, and designated it nagA. The nagA gene encoded a polypeptide of 600 amino acids with significant similarity to glucosaminidases and hexosaminidases of various eukaryotes. A. oryzae strain carrying the nagA gene under the control of the improved glaA promoter produced large amounts of β-N-acetylglucosaminidase in a wheat bran solid culture. The β-N-acetylglucosaminidase was purified from crude extracts of the solid culture by column chromatographies on Q-Sepharose and Sephacryl S-200. This enzyme was used for synthesis of lacto-N-triose II, which is contained in human milk. By reverse hydrolysis reaction, lacto-N-triose II and its positional isomer were synthesized from lactose and D-N-acetylglucosamine in 0.21% and 0.15% yield, respectively.
- 社団法人日本農芸化学会の論文
- 2003-03-23
著者
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Kitamoto Katsuhiko
Department of Biotechnology, The University of Tokyo
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Kim S
Korea Univ. Seoul Kor
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Nakajima Hiroo
京都府立医科大学 内分泌･乳腺外科学部門
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Nakajima Hiroo
Tsujigiwa Daigo Clinic 1-19 Daigo Shinmachi-uramachi Fushimi-ku Kyoto 601-1326 Jpn
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Kitamoto K
Univ. Tokyo Tokyo Jpn
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Kitamoto K
National Research Institute Of Brewing
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Kitamoto Katsuhiko
Dep. Of Biotechnology Graduate School Of Agricultural And Life Sciences The Univ. Of Tokyo
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NAKAJIMA Harushi
Department of Biotechnology, The University of Tokyo
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Matsuo I
Meiji Institute Of Health Science:(present Address)riken (institute Of Physical And Chemical Researc
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Kitamoto Katsuhiko
Department Of Biotechnology University Of Tokyo
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MATSUO Ichiro
Meiji Institute of Health Science
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KIM Sunhwa
Department of Biotechnology, University of Tokyo
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YAMAMOTO Yuichi
Meiji Institute of Health Science
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AJISAKA Katsumi
Meiji Institute of Health Science
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MARUYAMA Jun-ich
Department of Biotechnology, University of Tokyo
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Kim S
Department Of Biotechnology University Of Tokyo
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Ajisaka K
Meiji Inst. Health Sci. Odawara Jpn
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Ajisaka Katsumi
Meiji Dairies Co.
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Maruyama Jun-ich
Department Of Biotechnology University Of Tokyo
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Kitamoto Katsuhiko
National Research Institute Of Brewing:(present Address)department Of Biotechnology The University O
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Nakajima H
Department Of Endocrine Breast Surgery Kyoto Prefectural University Of Medicine
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Kitamoto Katsuhiko
Department Of Applied Biological Chemistry And Department Of Applied Biological Engineering Graduate
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Yamamoto Yuichi
Meiji Institute Of Health Science:(present Address)niigata University Of Pharmacy And Applied Life S
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Yamamoto Yuichi
Meiji Dairies Co.
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Nakajima Harushi
Department Of Agricultural Chemistry The University Of Tokyo
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Nakajima Hidenori
Exploratory Research Laboratories Fujisawa Pharmaceutical Co. Ltd
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Kimura K
Department Of Biomedical Sciences Graduate School Of Veterinary Medicine Hokkaido University
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