Development of a Versatile Expression Plasmid Construction System for Aspergillus oryzae and Its Application to Visualization of Mitochondria

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概要

• 論文の詳細を見る

• We report here a development of the MultiSite GatewayTM-based versatile plasmid construction system applicable for the rapid and efficient preparation of Aspergillus oryzae expression plasmids. This system allows the simultaneous connection of the three DNA fragments inserted in entry clones along with a destination vector in a defined order and orientation. We prepared a variety of entry clones and destination vectors containing promoters, genes encoding carrier-proteins and fusion tags, and selectable markers, which makes it possible to generate 80 expression plasmids for each target protein. Using this system, plasmids for expression of the EGFP fused with the mitochondrial-targeting signal of citrate synthase (AoCit1) were generated. Tubular structures of mitochondria were visualized in the transformants expressing the AoCit1-EGFP fusion protein. This plasmid construction system allows us to prepare a large number of expression plasmids without laborious DNA manipulations, which would facilitate molecular biological studies on A. oryzae.

• 社団法人 日本農芸化学会の論文
• 2006-08-23

著者

• Kitamoto Katsuhiko

  Department of Biotechnology, The University of Tokyo

• Arioka Manabu

  Department Of Biotechnology University Of Tokyo

• Arioka M

  Department Of Biotechnology The University Of Tokyo

• Arioka Manabu

  東京大学

• Arioka Manabu

  Department Of Biotechnology The University Of Tokyo

• Kitamoto K

  Univ. Tokyo Tokyo Jpn

• Kitamoto K

  National Research Institute Of Brewing

• Kitamoto Katsuhiko

  Dep. Of Biotechnology Graduate School Of Agricultural And Life Sciences The Univ. Of Tokyo

• MABASHI Yuka

  Department of Biotechnology, The University of Tokyo

• KIKUMA Takashi

  Department of Biotechnology, The University of Tokyo

• MARUYAMA Jun-ichi

  Department of Biotechnology, The University of Tokyo

• Maruyama J

  Dep. Of Biotechnology Graduate School Of Agricultural And Life Sciences The Univ. Of Tokyo

• Kikuma Takashi

  Department Of Biotechnology The University Of Tokyo

• Kitamoto Katsuhiko

  Department Of Biotechnology University Of Tokyo

• Maruyama Jun-ichi

  Dep. Of Biotechnology Graduate School Of Agricultural And Life Sciences The Univ. Of Tokyo

• Kitamoto Katsuhiko

  National Research Institute Of Brewing:(present Address)department Of Biotechnology The University O

• Mabashi Yuka

  Department Of Biotechnology The University Of Tokyo

• Kitamoto Katsuhiko

  Department Of Applied Biological Chemistry And Department Of Applied Biological Engineering Graduate

• Maruyama Jun-ichi

  Department Of Biotechnology Graduate School Of Agricultural And Life Sciences The University Of Toky

• Kimura K

  Department Of Biomedical Sciences Graduate School Of Veterinary Medicine Hokkaido University

• Maruyama Junichi

  Department Of Biotechnology The University Of Tokyo

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