Isolation and Characterization of a 230 kDa Protein (p230) Specifically Expressed in Fetal Brains: Its Involvement in Neurite Outgrowth from Rat Cerebral Cortex Neurons Grown on Monolayer of Astrocytes.

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概要

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• By screening with monoclonal antibodies (mAbs) raised against growth cone membrane fraction from fetal porcine brains, we have identified a 230kDa antigen, termed p230. Western blot analysis of extracts from various tissues demonstrated that p230 is specifically expressed in brains, in which its expression is temporally restricted; it was especially prominent in the embryonic and the early postnatal stage, and decreased to subdetectable levels in the adult brain. Further characterization of p230 revealed that it is a peripherally-membrane associated, cell surface protein produced by astrocytes. Neurite outgrowth of E18 rat cerebral cortex neurons cultured on a monolayer of astrocytes was significantly reduced in the presence of anti-p230 polyclonal antibody. Partial amino acid sequences of p230 purified from fetal porcine brains were highly homologous to an extracellular matrix protein, tenascin-C. These lines of evidence suggest that p230, a tenascin-C-like molecule present in fetal porcine brains, plays important roles during early brain development, particularly in growth cone guidance.

• 社団法人 日本生化学会の論文

著者

• Arioka Manabu

  Department Of Biotechnology The University Of Tokyo

• Ho Shih-ching

  Department Of Bioengineering Tatung Institute Of Technology

• Wakatsuki Shuji

  Department Of Biotechnology University Of Tokyo:(present Address)department Of Growth Regulation Ins

• Kitamoto Katsuhiko

  Department Of Applied Biological Chemistry And Department Of Applied Biological Engineering Graduate

• Yamasaki Makari

  College of Bioresource Science, Department of Food Science and Technology, Nihon University

• Yamasaki Makari

  College of Bioresource Sciences, Department of Food Science and Technology, Nihon University

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