Cloning and Characterization of the nagA Gene that Encodes β-N-Acetylglucosaminidase from Aspergillus nidulans and Its Expression in Aspergillus oryzae(Microbiology & Fermentation Technology)

スポンサーリンク

概要

• 論文の詳細を見る

• We isolated a β-N-acetylglucosaminidase encoding gene and its cDNA from the filamentous fungus Aspergillus nidulans, and designated it nagA. The nagA gene contained no intron and encoded a polypeptide of 603 amino acids with a putative 19-amino acid signal sequence. The deduced amino acid sequence was very similar to the sequence of Candida albicans Hexl and Trichoderma harzianum Nag1. Yeast cells containing the nagA cDNA under the control of the GAL1 promoter expressed β-N-acetylglucosaminidase activity. The chromosomal nagA gene of A. nidulans was disrupted by replacement with the argB marker gene. The disruptant strains expressed low levels of β-N-acetyl-glucosaminidase activity and showed poor growth on a medium containing chitobiose as a carbon source. Aspergillus oryzae strain carrying the nagA gene under the control of the improved glaA promoter produced large amounts of β-N-acetylglucosaminidase in a wheat bran solid culture.

• 社団法人日本農芸化学会の論文
• 2002-10-23

著者

• Kitamoto Katsuhiko

  Department of Biotechnology, The University of Tokyo

• Kim S

  Korea Univ. Seoul Kor

• Nakajima Hiroo

  京都府立医科大学 内分泌･乳腺外科学部門

• Nakajima Hiroo

  Tsujigiwa Daigo Clinic 1-19 Daigo Shinmachi-uramachi Fushimi-ku Kyoto 601-1326 Jpn

• Kitamoto K

  Univ. Tokyo Tokyo Jpn

• Kitamoto K

  National Research Institute Of Brewing

• Kitamoto Katsuhiko

  Dep. Of Biotechnology Graduate School Of Agricultural And Life Sciences The Univ. Of Tokyo

• NAKAJIMA Harushi

  Department of Biotechnology, The University of Tokyo

• Matsuo I

  Meiji Institute Of Health Science:(present Address)riken (institute Of Physical And Chemical Researc

• Kitamoto Katsuhiko

  Department Of Biotechnology University Of Tokyo

• MATSUO Ichiro

  Meiji Institute of Health Science

• KIM Sunhwa

  Department of Biotechnology, University of Tokyo

• AJISAKA Katsumi

  Meiji Institute of Health Science

• Kim S

  Department Of Biotechnology University Of Tokyo

• Ajisaka K

  Meiji Inst. Health Sci. Odawara Jpn

• Ajisaka Katsumi

  Meiji Dairies Co.

• Kitamoto Katsuhiko

  National Research Institute Of Brewing:(present Address)department Of Biotechnology The University O

• Nakajima H

  Department Of Endocrine Breast Surgery Kyoto Prefectural University Of Medicine

• Kitamoto Katsuhiko

  Department Of Applied Biological Chemistry And Department Of Applied Biological Engineering Graduate

• Nakajima Harushi

  Department Of Agricultural Chemistry The University Of Tokyo

• Nakajima Hidenori

  Exploratory Research Laboratories Fujisawa Pharmaceutical Co. Ltd

• Kimura K

  Department Of Biomedical Sciences Graduate School Of Veterinary Medicine Hokkaido University

関連論文

• Elevated Expression of Genes under the Control of Stress Response Element (STRE) and Msn2p in an Ethanol-Tolerance Sake Yeast Kyokai No. 11(BREWING AND FOOD TECHNOLOGY)
• A Eukaryotic Copper-Containing Nitrite Reductase Derived from a NirK Homolog Gene of Aspergillus oryzae
• Cloning of Aspergillus oryzae Aovps5 gene, homologous to vacuolar protein sorting associated gene VPS5 and construction of the disruptant(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
• Enhancement of paclitaxel-induced apoptosis in HER2-overexpressing human breast cancer cells by a pertuzumab mimetic peptide, HRAP(MEDICAL BIOTECHNOLOGY)
• S19159, a Modulator of Neurite Outgrowth Produced by the Ascomycete Preussia aemulans II. Structure Elucidation
• Purification and Characterization of Termite Endogenous β-1,4-Endoglucanases Produced in Aspergillus oryzae
• Multidrug Resistance Phenotype Conferred by Overexpressing bfr2^+/pad1^+/sks1^+or pap1^+Genes and Mediated by bfr1^+Gene Product, a Structural and Functional Homologue of P-Glycoprotein in Schizosaccharomyces pombe
• Development of a Versatile Expression Plasmid Construction System for Aspergillus oryzae and Its Application to Visualization of Mitochondria
• Adenine Auxotrophic Mutants of Aspergillus oryzae : Development of a Novel Transformation System with Triple Auxotrophic Hosts
• Identification and Characterization of Scpl5, a Protein from Streptomyces coelicolor A3(2) Inducing Neurites in PC12 Cells(Biochemistry & Molecular Biology)
• Functional Expression in Aspergillus oryzae of p15, a Protein with Potent Neurite-inducing Activity in PC12 Cells(Microbiology & Fermentation Technology)
• Characterization of a Novel Fungal Protein, p15, Which Induces Neuronal Differentiation of PC12 Cells
• S19159, a Modulator of Neurite Outgrowth Produced by the Ascomycete Preussia aemulans I. Producing Strain, Fermentation, Isolation and Biological Activity
• Identification and Characterization of Porcine NP-190, a Novel Protein That Is Specifically Expressed in the Axonal Membrane during the Embryonic Period^1
• Isolation and Characterization of a 230 kDa Protein(p230) Specifically Expressed in Fetal Brains: Its Involvement in Neurite Outgrowth from Rat Cerebral Cortex Neurons Grown on Monolayer of Astrocytes
• Hop-Resistant Lactobacillus brevis Contains a Novel Plasmid Harboring a Multidrug Resistance-Like Gene
• 3E10-5 Effect of disruption of the Aopexll genes involved in peroxisome biogenesis on Woronin body formation in Aspergillus oryzae
• Identification and Characterization of an Intracellular Lectin, Calnexin, from Aspergillus oryzae Using N-Glycan-Conjugated Beads
• Development of a Modified Positive Selection Medium That Allows to Isolate Aspergillus oryzae Strains Cured of the Integrated niaD-Based Plasmid
• Visualizing Nuclear Migration during Conidiophore Development in Aspergillus nidulans and Aspergillus oryzae : Multinucleation of Conidia Occurs through Direct Migration of Plural Nuclei from Phialides and Confers Greater Viability and Early Germination i
• Effects of Protein Transport Inhibitors on the Distribution and Secretion of the Fusion Protein RntA-EGFP in Aspergillus oryzae
• 2F14-4 Visualization and structural analysis of septal pore in Aspergillus oryzaev
• In vivo Visualization of the Distribution of a Secretory Protein in Aspergillus oryzae Hyphae Using the RntA-EGFP Fusion Protein(Microbiology & Fermentation Technology)
• Visualization of Nuclei in Aspergillus oryzae with EGFP and Analysis of the Number of Nuclei in Each Conidium by FACS(Microbiology & Fermentation Technology)
• Production and Product Quality Assessment of Human Hepatitis B Virus Pre-S2 Antigen in Submerged and Solid-State Cultures of Aspergillus oryzae
• Temperature-Dependent Gate Effect of Sintered HgTe Nanoparticles
• Synthesis of Single Crystalline In_2O_3 Nanowires and Their Photoluminescence Characteristics
• Photoresponse of a single ZnO nanowire illuminated by modulated light
• Photocurrent of Single Silicon Nanowire synthesized by Thermal Chemical Vapor Deposition
• Photocurrent and Photoluminescence Characteristics of Networked GaN Nanowires
• Development of HER2-antagonistic peptides as novel anti-breast cancer drugs by in silico methods
• Structural and Optical Properties of GaN Fine Particles Prepared from GaOOH
• Temperature Dependence of Photoluininescence of α-Ga_2O_3 Powders
• Brewing Properties of Sake Yeast Whose EST2 Gene Encoding Isoamyl Acetate-Hydrolyzing Esterase Was Disrupted
• Molecular Cloning and Nucleotide Sequence of the Isoamyl Acetate-Hydrolyzing Esterase Gene (EST2) from Saccharomyces cerevisiae : The Mechanism of Isoamyl Acetate Production by Sake Yeast (IV)
• Improvement of Heterologous Protein Production in Aspergillus oryzae by RNA Interference with α-Amylase Genes
• Cloning and Overexpression of β-N-Acetylglucosaminidase Encoding Gene nagA from Aspergillus oryzae and Enzyme-catalyzed Synthesis of Human Milk Oligosaccharide(Microbiology & Fermentation Technology)
• Cloning and Characterization of the nagA Gene that Encodes β-N-Acetylglucosaminidase from Aspergillus nidulans and Its Expression in Aspergillus oryzae(Microbiology & Fermentation Technology)
• The Effect of Pyruvate Decarboxylase Gene Knockout in Saccharomyces cerevisiae on L-Lactic Acid Production
• D-Lactic Acid Production by Metabolically Engineered Saccharomyces cerevisiae(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
• Fas Ligand Released by Activated Monocytes Causes Apoptosis of Lung Epithelial Cells in Human Acute Lung Injury Model in Vitro(Molecular and Cell Biology)
• Insertion Analysis of Putative Functional Elements in the Promoter Region of the Aspergillus oryzae Taka-amylase A Gene (amyB) Using a Heterologous Aspergillus nidulans amdS-lacZ Fusion Gene System
• Characteristic Expression of Three Amylase-encoding Genes, agdA, amyB, and glaA in Aspergillus oryzae Transformants Containing Multiple Copies of the agdA Gene
• A Case of Non-Metastatic Giant Mucinous Carcinoma of the Breast
• Aberrant Expression of Fra-1 in Estrogen Receptor-negative Breast Cancers and Suppression of their Propagation In Vivo by Ascochlorin, an Antibiotic that Inhibits Cellular Activator Protein-1 Activity
• Biosyntheses of Sescandelin and Sescandelin B : New Isocoumarin Compounds Produced by the Fungus, Sesquicilium candelabrum
• Importance of the Ketone Function for the Phytotoxicity of Spiciferone A, a Phytotoxin Produced by the Fungus Cochliobolus spicifer
• BSF-A, a New Plant Growth Regulator Produced by the Fungus, Botrytis squamosa
• Dihydroampullicin, a New Plant Growth Regulators Produced by the Ampulliferina-like Fungus sp. No.27
• Altechromones A and B, New Plant Growth Regulators Produces by the Fungus, Alternaria sp.
• Absolute Stereochemistry of Neovasinin, a Phytotoxin Produced by the Fungus, Neocosmospora vasinfecta
• Structure and Biological Activity of Plant Growth Regulators Produced by Penicillium
• Hericerin, a New Pollen Growth Inhibitor from the Mushroom Hericium erinaceum(Organic Chemistry)
• Structure of Sescandelin-B, a New Metabolite Produced by the Fungus, Sesquicillium candelabrum(Organic Chemistry)
• Vulculic Acid, a Pollen Germination Inhibitor Produced by the Fungus, Penicillium sp.(Organic Chemistry)
• Sescandelin, a New Root Promoting Substance Produced by the Fungus, Sesquicillium candelabrum(Organic Chemistry)
• Ampullicin and Isoampullicin, New Metabolites from an Ampulliferina-like Fungus sp. No.27(Organic Chemistry)
• Structure of Spiciferone A, a Novel γ-Pyrone Plant Growth Inhibitor Produced by the Fungus Cochliobolus spicifer Nelson(Organic Chemistry)
• Production of Cyclosporin by Fungi Belonging to the Genus Neocosmospora(Biological Chemistry)
• Structure of Rosellichalasin, a New Metabolite Produced by Rosellinia necatrix(Organic Chemistry)
• Isolation and Structures of O-Methyldihydrobotrydial and Deacetyl-O-methyldihydrobotrydialone Produced by Botrytis squamosa(Organic Chemistry)
• Isolation of 2-Acetylamino-3-hydroxy-4-methyloct-6-enoic Acid, a Derivative of the "C_9-Amino Acid" Residue of Cyclosporins, Produced by the Fungus Neocosmospora vasinfecta E. F. Smith(Organic Chemistry)
• Apoptosis of Endothelial Cell Line ECV304 Persistently infected with Orientia tsutsugamushi
• Immunosuppressive Activity of 4-O-Methylascochlorin
• Elimination of Cell-cycle Regulators during Caspase-3-dependent Apoptosis Caused by an Immunosuppressant, FTY720(Biochemistry & Molecular Biology)
• Transport of Charge Carriers in HgTe/CdTe Core-Shell Nanoparticle Film
• Structural and Optical Properties of ZnO Nanowires Synthesized from Ball-Milled ZnO Powders
• A New Antimitotic Substance, FR182877 : II. The Mechanism of Action
• Molecular Cloning,Expression,and Characterization of a Novel Mouse Liver SULT1B1 Sulfotransferase
• cDNA Cloning, Expression, and Characterization of the Human Bifunctional ATP Sulfurylase/Adenosine 5'-Phosphosulfate Kinase Enzyme
• New Antitumor Substances, FR901463, FR901464 and FR901465 II. Activities against Experimental Tumors in Mice and Mechanism of Action
• Control with Organic Solvents of Efficiency of Persolvent Cholesterol Fermentation by Pseudomonas sp. Strain ST-200
• Purification and Characterization of Adenosine 5'-Phosphosulfate Kinase from the Thermophilic Bacterium Bacillus stearothermophilus
• soxRS Gene Increased the Level of Organic Solvent Tolerance in Escherichia coli
• A Close Correlation between Improvement of Organic Solvent Tolerance Levels and Alteration of Resistance toward Low Levels of Multiple Antibiotics in Escherichia coli
• Action of FR901228, a Novel Antitumor Bicyclic Depsipeptide Produced by Chromobacterium violaceum No. 968, on Ha-ras Transformed NIH3T3 Cells
• Detection of the Cholera Toxin-binding Activity of κ-Casein Macropeptide and Optimization of Its Production by the Response Surface Methodology
• Isolation, X-Ray Structure and Biological Activity of Deacetyl-dihydrobotrydial Produced by Botrytis squamosa
• Aspergillus oryzae palB^ Encodes a Calpain-Like Protease : Homology to Emericella nidulans PalB and Conservation of Functional Regions
• Cloning and Characterization of the cpyA Gene Encoding Intracellular Carboxypeptidase from Aspergillus nidulans
• Property of Taka-amylase A with Glu or Asp of the Catalytic Residue Replaced by the Corresponding Amine
• Genetic Analysis of Growth Inhibition of Yeast Cells Caused by Expression of Aspergillus oryzae RNase T1
• Monitoring Global Gene Expression of Proteases and Improvement of Human Lysozyme Production in the nptB Gene Disruptant of Aspergillus oryzae
• Expression of an 87-kD-β-1,3-Glucanase of Bacillus circulans IAM1165 in Saccharomyces cerevisiae by Low-temperature Incubation
• Preparation of 3'-Phosphoadenosine 5'-Phospho^S-sulfate Using ATP Sulfurylase and APS Kinase from Bacillus stearothermophilus: Enzymatic Synthesis and Purification
• Effective Isolation and Identification of Toluene-tolerant Pseudomonas Strains
• Structures of Naphthoquinones Produced by the Fungus, Fusarium sp., and Their Biological Activity toward Pollen Germination(Organic Chemistry)
• Structure of Neovasinone, a New α-Pyrone Plant Growth Regulator Produced by the Fungus, Neocosmospora vasinfecta E. F. Smith(Organic Chemistry)
• Cloning and Characterization of the bipA Gene Encoding ER Chaperone BiP from Aspergillus oryzae
• Cloning, Characterization and Overexpression of a Gene (pdiA) Encoding Protein Disulfide Isomerase of Aspergillus oryzae
• Efficient Hardware-Software Partitioning for a Digital Dental X-Ray System
• Genomics of Aspergillus oryzae
• A Eukaryotic Copper-Containing Nitrite Reductase Derived from a NirK Homolog Gene of Aspergillus oryzae
• Identification and Characterization of an Intracellular Lectin, Calnexin, from Aspergillus oryzae Using N-Glycan-Conjugated Beads
• Improvement of Heterologous Protein Production in Aspergillus oryzae by RNA Interference with α-Amylase Genes
• Light Represses Conidiation in Koji Mold Aspergillus oryzae
• Purification and Characterization of Termite Endogenous β-1,4-Endoglucanases Produced in Aspergillus oryzae
• Monitoring Global Gene Expression of Proteases and Improvement of Human Lysozyme Production in the nptB Gene Disruptant of Aspergillus oryzae
• A Study on a Road-Adaptive CW / CA Algorithm for Automobiles Using HiL Simulations
• Stereochemistry and Biological Activities of LL-P880γ, a Pestalotin Analogue, Produced by Penicillium citreo-viride

もっと見る 閉じる

スポンサーリンク