Functional Expression in Aspergillus oryzae of p15, a Protein with Potent Neurite-inducing Activity in PC12 Cells(Microbiology & Fermentation Technology)
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概要
- 論文の詳細を見る
We previously reported that a fungal protein, p15, induces neurite outgrowth and differentiation of rat pheochromocytoma PC12 cells through the activation of the Ca^<2+> signaling pathway. We report here the secretory production of p15 in Aspergillus oryzae. Analysis of culture supernatant of A. oryzae transformed with the gene encoding the p15 precursor tagged with a hemagglutinin (HA) epitope demonstrated that the transformant secreted a protein with an apparent molecular mass of 17.5 kDa, which is a little larger than the expected size of mature p15-HA. By heat denaturation and ion exchange chromatography, p15-HA was easily purified from the culture supernatant with sufficient abundance. Although purified p15-HA was less active than the native p15 obtained from the culture broth of a producing fungal strain, it had neurite-inducing activity in PC12 cells in a dose-dependent manner, providing a system to study the action mechanism of p15.
- 社団法人日本農芸化学会の論文
- 2002-03-23
著者
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Kitamoto Katsuhiko
Department of Biotechnology, The University of Tokyo
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Arioka Manabu
Department Of Biotechnology University Of Tokyo
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Arioka M
Department Of Biotechnology The University Of Tokyo
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Arioka Manabu
東京大学
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Arioka Manabu
Department Of Biotechnology The University Of Tokyo
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Kitamoto K
Univ. Tokyo Tokyo Jpn
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Kitamoto K
National Research Institute Of Brewing
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Kitamoto Katsuhiko
Dep. Of Biotechnology Graduate School Of Agricultural And Life Sciences The Univ. Of Tokyo
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NAKASHIMA Satoru
Department of Biotechnology, University of Tokyo
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Kitamoto Katsuhiko
Department Of Biotechnology The University Of Tokyo
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Kitamoto Katsuhiko
Department Of Biotechnology University Of Tokyo
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Kitamoto Katsuhiko
National Research Institute Of Brewing:(present Address)department Of Biotechnology The University O
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Kitamoto Katsuhiko
Department Of Applied Biological Chemistry And Department Of Applied Biological Engineering Graduate
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Nakashima Sadahiko
Department Of Biotechnology University Of Tokyo
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Kimura K
Department Of Biomedical Sciences Graduate School Of Veterinary Medicine Hokkaido University
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