Electrophoretic Karyotype and Gene Assignment to Chromosomes of Aspergillus oryzae
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概要
- 論文の詳細を見る
An electrophoretic karyotype of Aspergillus oryzae was obtained by transverse altering-field ele-ctrophoresis. Seven chromosomal bands were found. With Schizosaccharomyces pombe chromosomes as size standards, we estimated the sizes of the chromosomes to be 7. 0, 5. 2, 5. 0, 4. 5, 4. 0, 3. 7, and 2. 8 megabase pairs (Mbp). The chromosomal DNA bands were identified with use of 13 cloned genes including ribosomal DNA. The intensity of ethidium staining and the results of Southern blotting with 100 random clones isolated from A. oryzae suggested that the smallest band migrated as doublet and that the total genome size was approximately 35Mbp.
- 社団法人日本農芸化学会の論文
- 1994-08-23
著者
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KIMURA Kazuhiro
National Institute for Materials Science
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Kitamoto K
Univ. Tokyo Tokyo Jpn
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Kitamoto K
National Research Institute Of Brewing
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Kitamoto Katsuhiko
Department Of Biotechnology University Of Tokyo
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Kitamoto Katsuhiko
National Research Institute Of Brewing
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GOMI Katsuya
National Research Institute of Brewing
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KUMAGAI Chieko
National Research Institute of Brewing
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Kumagai C
National Research Institute Of Brewing
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Gomi K
Tohoku Univ. Miyagi Jpn
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Kumagai Chieko
General Research Laboratories Ozeki Corp.
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Kimura K
Department Of Biomedical Sciences Graduate School Of Veterinary Medicine Hokkaido University
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