Deletion Analysis of the Taka-amylase A Gene Promoter Using a Homologous Transformation System in Aspergillus oryzae
スポンサーリンク
概要
- 論文の詳細を見る
The Taka-amylase A gene (amy B) of Aspergillus oryzae is induced by starch or maltose. The molecular mechanism of the induction was investigated using a fusion of the amy B promoter and the Escherichia coli uid A gene encoding β-glucuronidase (GUS). To identify the region responsible for high-level expression and regulation within the amy B promoter, a series of deletion promoters was constructed and introduced into the A. oryzae met locus by homologous recombination. Deletion of the region between -377 to -290 (the number indicates the distance in base pairs from the translation initiation point (+1) to the deletion end point) significantly reduced of the GUS activity, but slight reduction of the GUS activity was observed in deletions up to -377. Northern blot analysis showed that reduction of the GUS activity depended upon the expression level of the GUS gene. The region between -377 to -290 is suggested to include the sequence required directly for high-level expression and regulation of the amy B gene.
- 社団法人日本農芸化学会の論文
- 1992-11-23
著者
-
Kitamoto K
Univ. Tokyo Tokyo Jpn
-
Kitamoto K
National Research Institute Of Brewing
-
Tamura G
Department Of Agricultural Chemistry The University Of Tokyo
-
Tamura G
Research Institute Of Brewing Resources Co. Ltd.
-
Tamura Gakuzo
Research Institute Of Brewing Resources Co. Ltd.
-
Kitamoto Katsuhiko
Department Of Biotechnology University Of Tokyo
-
Kitamoto Katsuhiko
National Research Institute Of Brewing
-
GOMI Katsuya
National Research Institute of Brewing
-
Tamura Gakuzo
Nuclear Receptor Institute Ksp
-
KUMAGAI Chieko
National Research Institute of Brewing
-
Tsuchiya Kozo
Research Institute of Brewing Resources Co.
-
TADA Setsuzo
Research Institute of Brewing Resources Co., Ltd.
-
GOMI Katsuya
Research Institute of Brewing Resources Co., Ltd.
-
Kumagai C
National Research Institute Of Brewing
-
Tada Sumihiko
Department Of Marine Bioresources Chemistry Faculty Of Fisheries Hokkaido University
-
Gomi K
Tohoku Univ. Miyagi Jpn
-
Kumagai Chieko
General Research Laboratories Ozeki Corp.
-
Tsuchiya K
Nisshin Oil Mills Ltd. Yokohama‐shi Jpn
-
Kimura K
Department Of Biomedical Sciences Graduate School Of Veterinary Medicine Hokkaido University
関連論文
- S19159, a Modulator of Neurite Outgrowth Produced by the Ascomycete Preussia aemulans II. Structure Elucidation
- Multidrug Resistance Phenotype Conferred by Overexpressing bfr2^+/pad1^+/sks1^+or pap1^+Genes and Mediated by bfr1^+Gene Product, a Structural and Functional Homologue of P-Glycoprotein in Schizosaccharomyces pombe
- Development of a Versatile Expression Plasmid Construction System for Aspergillus oryzae and Its Application to Visualization of Mitochondria
- Adenine Auxotrophic Mutants of Aspergillus oryzae : Development of a Novel Transformation System with Triple Auxotrophic Hosts
- Identification and Characterization of Scpl5, a Protein from Streptomyces coelicolor A3(2) Inducing Neurites in PC12 Cells(Biochemistry & Molecular Biology)
- Functional Expression in Aspergillus oryzae of p15, a Protein with Potent Neurite-inducing Activity in PC12 Cells(Microbiology & Fermentation Technology)
- Characterization of a Novel Fungal Protein, p15, Which Induces Neuronal Differentiation of PC12 Cells
- S19159, a Modulator of Neurite Outgrowth Produced by the Ascomycete Preussia aemulans I. Producing Strain, Fermentation, Isolation and Biological Activity
- Identification and Characterization of Porcine NP-190, a Novel Protein That Is Specifically Expressed in the Axonal Membrane during the Embryonic Period^1
- Isolation and Characterization of a 230 kDa Protein(p230) Specifically Expressed in Fetal Brains: Its Involvement in Neurite Outgrowth from Rat Cerebral Cortex Neurons Grown on Monolayer of Astrocytes
- Hop-Resistant Lactobacillus brevis Contains a Novel Plasmid Harboring a Multidrug Resistance-Like Gene
- The Gene Responsible for Tunicamycin Resistance, tmrB, in Bacillus subtilis(Microbilolgy & Fermentation Industry)
- 3E10-5 Effect of disruption of the Aopexll genes involved in peroxisome biogenesis on Woronin body formation in Aspergillus oryzae
- Development of a Modified Positive Selection Medium That Allows to Isolate Aspergillus oryzae Strains Cured of the Integrated niaD-Based Plasmid
- Visualizing Nuclear Migration during Conidiophore Development in Aspergillus nidulans and Aspergillus oryzae : Multinucleation of Conidia Occurs through Direct Migration of Plural Nuclei from Phialides and Confers Greater Viability and Early Germination i
- Effects of Protein Transport Inhibitors on the Distribution and Secretion of the Fusion Protein RntA-EGFP in Aspergillus oryzae
- 2F14-4 Visualization and structural analysis of septal pore in Aspergillus oryzaev
- In vivo Visualization of the Distribution of a Secretory Protein in Aspergillus oryzae Hyphae Using the RntA-EGFP Fusion Protein(Microbiology & Fermentation Technology)
- Visualization of Nuclei in Aspergillus oryzae with EGFP and Analysis of the Number of Nuclei in Each Conidium by FACS(Microbiology & Fermentation Technology)
- Production and Product Quality Assessment of Human Hepatitis B Virus Pre-S2 Antigen in Submerged and Solid-State Cultures of Aspergillus oryzae
- Brefeldin A, a Specific Inhibitor of Intracellular Translocation of Vesicular Stomatitis Virus G Protein: Intracellular Accumulation of High-mannose Type G Protein and Inhibition of Its Cell Surface Expression
- Stereochemical Configuration of 1-1 Glycosidic Linkages in Tunicamycin
- Effect of Brefeldin A on Biosynthesis of Cellular Components in Candida albicans
- A New Screening Procedure on Selective Inhibitors against SV40-Transformed Cells
- PPARγ Activation and Adipocyte Differentiation Induced by AS-6, a Prenyl-phenol Antidiabetic Antibiotic
- Induction of Two Types of Gene Amplification by a Cloned DNA Fragment and Amplification of a Foreign Gene on the Bacillus subtilis Chromosome by Using the Fragment
- Construction of New ARSI Vectors with Increased Mitotic Stability in Saccharomyces cerevisiae(Microbiology & Fermentation Industry)
- Mutational Site in Tunicamycin-resistant Mutation tmrB8 of Bacillus subtilis as Revealed at the Nucleotide Sequence Level(Microbiology & Fermentation Industry)
- Efficacies of Different Secretion Vectors for Secretion of Human Epidermal Growth Factor by Escherichia coli(Biological Chemistry)
- Novel Saccharomyces cerevisiae Temperature-sensitive cyrl Mutant Which Accumulates Glycogen Particles
- recE-Dependent Gene Amplification Induced by a Tunicamycin-resistant Mutation (tmrA7) in Bacillus subtilis(Microbiology & Fermentation Industry)
- Expression and Secretion of Human Epidermal Growth Factor in Escherichia coli(Microbiology & Fermentation Industry)
- Sequence of the Signal Peptide of E. coli Alkaline Phosphatase
- Brewing Properties of Sake Yeast Whose EST2 Gene Encoding Isoamyl Acetate-Hydrolyzing Esterase Was Disrupted
- Molecular Cloning and Nucleotide Sequence of the Isoamyl Acetate-Hydrolyzing Esterase Gene (EST2) from Saccharomyces cerevisiae : The Mechanism of Isoamyl Acetate Production by Sake Yeast (IV)
- Construction of Uracil and Tryptophan Auxotrophic Mutants from Sake Yeasts by Disruption of URA3 and TRP1 Genes(Microbiology & Fermentation Industry)
- Cloning and Overexpression of β-N-Acetylglucosaminidase Encoding Gene nagA from Aspergillus oryzae and Enzyme-catalyzed Synthesis of Human Milk Oligosaccharide(Microbiology & Fermentation Technology)
- Cloning and Characterization of the nagA Gene that Encodes β-N-Acetylglucosaminidase from Aspergillus nidulans and Its Expression in Aspergillus oryzae(Microbiology & Fermentation Technology)
- Effect of Yeast Fumarase Gene (FUM1) Dsruption on Production of Malic, Fumaric and Succinic Acids in Sake Mash
- Molecular Cloning and Determination of the Nucleotide Sequence of a Gene Encoding an Acid-Stable α-Amylase from Aspergillus kawachii
- Construction of Urea Non-producing Yeast Saccharomyces cerevisiae by Disruption of the CAR1 Gene(Microbiology & Fermentation Industry)
- Differentiation of Mouse and Human Myeloid Leukemia Cells Induced by an Antitumor Antibiotic, Ascofuranone(Biological Chemistry)
- Digalactosyldiacylglycerol Suppression of Inhibition by Sulfoquinovosyldiacylglycerol of α-Glucosidase
- Insertion Analysis of Putative Functional Elements in the Promoter Region of the Aspergillus oryzae Taka-amylase A Gene (amyB) Using a Heterologous Aspergillus nidulans amdS-lacZ Fusion Gene System
- Characteristic Expression of Three Amylase-encoding Genes, agdA, amyB, and glaA in Aspergillus oryzae Transformants Containing Multiple Copies of the agdA Gene
- Nucleotide Sequence and Expression of α-GIucosidase-encoding Gene (agdA) from Aspergillus oryzae
- Cloning and Nucleotide Sequence of the Calmodulin-Encoding Gene (cmdA) from Aspergillus oryzae
- High Level Secretion of Calf Chymosin Using a Glucoamylase-prochymosin Fusion Gene in Aspergillus oryzae
- Deletion Analysis of the Taka-amylase A Gene Promoter Using a Homologous Transformation System in Aspergillus oryzae
- Identification of the Promoter Region of the Taka-amylase A Gene Required for Starch Induction(Microbiology & Fermentation Industry)
- The Glucoamylase cDNA from Aspergillus oryzae : Its Cloning, Nucleotide Sequence, and Expression in Saccharomyces cerevisiae(Microbiology & Fermentation Industry)
- Cloning and Nucleotide Sequence of the Alcohol Acetyltransferase II Gene (ATF2) from Saccharomyces cerevisiae Kyokai No.7
- Chromosomal Transformation in Saccharomyces cerevisiae with DNA Isolated by Pulse Field Gel Electrophoresis(Microbiology & Fermentation Industry)
- Stimulation of Insulin-dependent Glucose Transport and Lipogenesis in Isolated Rat Adipocytes by Trypsinized Soybean Glycinin Acidic Subunit A_
- Insulin-modulating Action of Glycinin Acidic Subunit A_ in Isolated Adipocytes Mimicking That of Bacitracin
- Overproduction of an α-Amylase/Glucoamylase Fusion Protein in Aspergillus oryzae Using a High Expression Vector
- Construction of an α-Amylase/Glucoamylase Fusion Gene and Its Expression in Saccharomyces cerevisiae
- Characteristics of Koji Prepared from the Transformant of Aspergillus oryzae with the Glucoamylase Gene of Aspergillus shirousamii, and Its Utilization for Sake-Brewing
- Cloning of the α-Amylase cDNA of Aspergillus shirousamii and Its Expression in Saccharomyces cerevisiae
- Immunological Comparison of Nitrite Reductase from Different Plant Sources
- Electron Donor for Nitrite Reductase from Etiolated Bean Stems
- Some Properties of Ferredoxin-nitrite Reductase from Spinacia oleracea
- Characterization of Substances that Restore Impaired Cell Division of UV-Irradiated E. coli B
- Antitumor Activity of 5-Hydroxymethyl-2-furoic Acid
- Functional Role of Aspergillus oryzae Glucoamylase C-Terminal Domain Investigated Using Its cDNA
- Molecular Cloning of the Glucoamylase Gene of Aspergillus shirousami and Its Expression in Aspergillus oryzae(Microbiology & Fermentation Industry)
- Cloning and Nucleotide Sequence of the Genomic Taka-amylase A Gene of Aspergillus oryzae(Microbiology & Fermentation Industry)
- Cloning and Nucleotide Sequence of the Ribonuclease T_1 Gene (rntA) from Aspergillus oryzae and Its Expression in Saccharomyces cerevisiae and Aspergillus oryzae
- Molecular Cloning and Characterization of a Transcriptional Activator Gene, amyR, Involved in the Amylolytic Gene Expression in Aspergillus oryzae
- Molecular Cloning of a Genomic DNA for Enolase from Aspergillus oryzae
- Isolation of a New Lytic Enzyme for Hiochi Bacteria and Other Lactic Acid Bacteria
- Identification and Characterization of an Intracellular Lectin, Calnexin, from Aspergillus oryzae Using N-Glycan-Conjugated Beads
- Cloning and Functional Analysis of the Aspergillus oryzae Conidiation Regulator Gene brlA by Its Disruption and Misscheduled Expression
- 904 Isolation and Characterization of Mutants of Saccharomyces cerevisiae with pH-sensitive Growth
- Breeding of a New Type of Baker's Yeast by δ-Integration for Overoproduction of Glucoamylase Using a Homothallic Yeast
- Electrophoretic Karyotype and Gene Assignment to Chromosomes of Aspergillus oryzae
- A Novel Culture Method for High Level Production of Heterologous Protein in Saccharomyces cerevisiae
- Cloning and Nucleotide Sequence of the Acid Protease-encoding Gene (pepA) from Aspergillus oryzae
- Mutant Isolation of Non-Urea Producing Sake Yeast by Positive Selection
- Transformation of the Industrial Strain of Aspergillus oryzae with the Homologous amdS Gene as a Dominant Selectable Marker
- Site-directed Mutagenesis of Catalytic Active-site Residues of Taka-amylase A
- Improvement of Heterologous Protein Production in Aspergillus oryzae by RNA Interference with α-Amylase Genes
- Rapid and Sensitive Detection of Hiochi Bacteria by Amplification of Hiochi Bacterial Common Antigen Gene by PCR Method and Characterization of the Antigen
- Production of Fungal Cell Wall Lytic Enzymes by Oerskovia sp. CK and Some Propertics of the Crude Enzymes.
- Transformation System for Aspergillus oryzae with Double Auxotrophic Mutations, niaD and sC
- Transformation of Aspergillus oryzae through Plasmid-mediated Complementation of the Methionine-auxotrophic Mutation(Microbiology & Fermentation Industry)
- L-Canavanine Resistance as a Positive Selectable Marker in Diploid Yeast Transformation through Integral Disruption of the CAN1 Gene
- Identification of a Heat-Stable Precursor of Dimethyl Sulfide in Sake