Molecular Cloning and Determination of the Nucleotide Sequence of a Gene Encoding an Acid-Stable α-Amylase from Aspergillus kawachii
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概要
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Complementary and genomic DNAs encoding an acid-stable α-amylase (asAA) were cloned from Aspergillus kawachii IFO4308 and their nucleotide sequences were determined. The structural gene (asaA) consisted of a 1,920 bp open reading frame that encoded 640 amino acids. The gene consisted of 9 exons and 8 introns. The signal peptide was composed of 21 amino acid residues. The amino acid sequence from the N-terminus to the 479th residue showed 97% homology with the Aspergillus niger acid α-amylase and that from the 480th residue to the C-terminus, including the raw-starch-affinity site, i.e., the TS region, the amino acid sequence of which was rich in threonine and serine, was characteristic of the asAA. A yeast transformant carrying the cDNA produced and secreted an acid-stable α-amylase which was capable of digesting raw starch.
- 社団法人日本生物工学会の論文
- 1996-04-25
著者
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Tamura Gakuzo
Research Institute Of Brewing Resources Co. Ltd.
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Kaneko Akihiro
Research Institute Of Brewing Resources Co. Ltd. :(present Address)wines & Spirits Technical Res
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SUDO SHIGETOSHI
National Research Institute of Brewing
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TAKAYASU-SAKAMOTO YUKO
National Research Institute of Brewing
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ISHIKAWA TAKEAKI
National Research Institute of Brewing
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OBA TOSHITERU
National Research Institute of Brewing
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TAMURA GAKUZO
Research Institute of Brewing Resources Co. Ltd.,
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