Isolation and Characterization of Starch-utilizing Mutants of Escherichia coli
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概要
- 論文の詳細を見る
Starch-utilizing mutants of Escherichia coli which can grow well on starch or amylose as the sole carbon source were isolated. The maximal viable cell number of the starch-utilizing mutants on the polysaccharide media reached the same level (4×109 cells/ml) as that with glucose medium after incubation for 24 hours at 37°C. The isolated mutants could produce more intracellular α-amylase than the wild-type strain, and the enzyme activity was detected in the extracellular fluid. Polyacrylamide gel electrophoresis showed that the intracellular and extracellular enzymes had similar electrophoretic mobilities. These observations suggested that the ability of growth on the polysaccharide media was due to the excreted α-amylase, which appeared to be identical with the intracellular enzyme.
- 社団法人 日本農芸化学会の論文
著者
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Iimura Yuzuru
National Research Institute of Brewing
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Morikawa Minoru
Institute For Production Research And Development The Nikka Whiskey Distilling Co. Ltd.
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ISHIKAWA TAKEAKI
National Research Institute of Brewing
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Shibuya Ichiro
Institute For Production Research And Development The Nikka Distilling Co. Ltd.
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Ouchi Kozo
National Research Institute Of Brewing
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NISHIYA TAKAMICHI
National Research Institute of Brewing
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MATSUYAMA Asahi
Life Science Research Laboratory, Kikkoman Corporation
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YAMAMOTO Tetsuro
Technological Department, Takara Shuzo Corporation
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