Yeast Transformation without the Spheroplasting Process
スポンサーリンク
概要
- 論文の詳細を見る
A plasmid, YRp7, carrying the yeast TRP1 gene transformed a trpl strain JH of Saccharomyces cerevisiae without spheroplasting. The transformation conditions were that intact cells were incubated with the plasmid DNA in chilled CaCl2 solution and then the incubation temperature was raised. The transformation frequency changed markedly with the CaCl2 concentration of the solution and the temperature rise after incubation at 0°. The optimal CaCl2 concentration and temperature for strain JH were 200 mM and 37°, respectively. Polyethylene glycol and the age of cells showed little effect on the transformation. The highest transformation frequency obtained was 300 colonies per μg plasmid DNA, and this was comparable to that obtained with the JH strain by the conventional method using spheroplasts. Transformation was confirmed genetically.
- 社団法人 日本農芸化学会の論文
著者
-
Iimura Yuzuru
National Research Institute of Brewing
-
Ouchi Kozo
National Research Institute Of Brewing
-
NISHIYA TAKAMICHI
National Research Institute of Brewing
-
GOTOH Kuniyasu
National Research Institute of Brewing
関連論文
- Isolation and Characterization of a Yeast Cryptococcus sp. S-2 That Produces Raw Starch-digesting α-Amylase, Xylanase, and Polygalacturonase
- Regulation of Isoamyl Alcohol Formation via Ehrlich Pathwas in Saccharomyces cerevisiae
- Effect of Yeast Fumarase Gene (FUM1) Dsruption on Production of Malic, Fumaric and Succinic Acids in Sake Mash
- Construction of Urea Non-producing Yeast Saccharomyces cerevisiae by Disruption of the CAR1 Gene(Microbiology & Fermentation Industry)
- Development of an Integrative DNA Transformation System for the Yeast Hansenula anomala
- UV-Killed Protoplast Fusion as a Method for Breeding Killer Yeasts
- Molecular Cloning of the Glucoamylase Gene of Aspergillus shirousami and Its Expression in Aspergillus oryzae(Microbiology & Fermentation Industry)
- Cloning and Nucleotide Sequence of the Genomic Taka-amylase A Gene of Aspergillus oryzae(Microbiology & Fermentation Industry)
- Acid Xylanase from Yeast Cryptococcus sp. S-2: Purification, Characterization, Cloning, and Sequencing
- Molecular Cloning of CWP1: A Gene Encoding a Saccharomyces cerevisiae Cell Wall Protein Solubilized with Rarobacter faecitabidus Protease I
- Productino of HM-1 Killer Toxin in Saccharomyces cerevisiae Transformed with the PDR4 Gene and δ-Sequence-Mediated Multi-Integration System
- Structural Relationships among Killer Toxins Secreted from the Killer Strains of the Genus Williopsis
- Isolation and Nucleotide Sequence of Hansenula anomala URA3 Gene Encoding Orotidine-5'-Phosphate Decarboxylase
- Changes in Sulfur Compounds of Awamori during Aging : Studies on the Maturation of Awamori(VIII)
- Changes in Ester Compounds and Higher Alcohols of Awamori during Aging : Studies on the Maturation of Awamori (VII)
- Changes in Free Fatty Acids of Awamori during Aging : Studies on the Maturation of Awamori (V)
- Molecular Cloning and Characterization of a Transcriptional Activator Gene, amyR, Involved in the Amylolytic Gene Expression in Aspergillus oryzae
- Cloning and Functional Analysis of the Aspergillus oryzae Conidiation Regulator Gene brlA by Its Disruption and Misscheduled Expression
- The Breeding of Cryophilic Killer Wine Yeasts
- Production of Fungal Cell Wall Lytic Enzymes by Oerskovia sp. CK and Some Propertics of the Crude Enzymes.
- Transformation of Aspergillus oryzae through Plasmid-mediated Complementation of the Methionine-auxotrophic Mutation(Microbiology & Fermentation Industry)
- Relationship between the Preference for Sake (Japanese rice wine) and the Movements of Metabolic Parameters Coinciding with Sake Intake
- L-Canavanine Resistance as a Positive Selectable Marker in Diploid Yeast Transformation through Integral Disruption of the CAN1 Gene
- Isolation of Uracil Auxotrophic Mutants of Saccharomyces cerevisiae Unable to Reduce 2, 3, 5-Triphenyltetrazolium Chloride
- Isolation and Characterization of Starch-utilizing Mutants of Escherichia coli
- Yeast Transformation without the Spheroplasting Process
- Fatty Acids Associated with the Cell Wall in Film Strains of Saccharomyces