UV-Killed Protoplast Fusion as a Method for Breeding Killer Yeasts

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概要

• 論文の詳細を見る

• A simple method for breeding killer yeasts without changing the nuclear genotype was described. Killer plasmids were introduced into recipient cells just after killing the protoplasts carrying killer plasmids with UV-rays. For the donors, UV-sensitive strains harboring killer plasmids having 100 base pair deletion and drug resistant mitochondria were constructed. Almost all fusants obtained showed the same nuclear phenotype as the recipient but had killer activity and drug resistance. Killer sake yeast were bred using a commercial strain, Kyokai 7,as the recipient, and they were confirmend to produce sake of the same quality as that produced with strain Kyokai 7 with no contamination by wild yeast.

• 社団法人日本生物工学会の論文
• 1983-12-25

著者

• OUCHI KOZO

  National Research Institute of Brewing, National Tax Administration

• AKIYAMA HIROICHI

  National Research Institute of Brewing, National Tax Administration

• Ouchi Kozo

  National Research Institute Of Brewing

• NISHITA TAKAMICHI

  National Research Institute of Brewing

• Akiyama Hiroichi

  National Research Institute Of Brewing

関連論文

• Regulation of Isoamyl Alcohol Formation via Ehrlich Pathwas in Saccharomyces cerevisiae
• UV-Killed Protoplast Fusion as a Method for Breeding Killer Yeasts
• Isolation of Uracil Auxotrophic Mutants of Saccharomyces cerevisiae Unable to Reduce 2, 3, 5-Triphenyltetrazolium Chloride
• Isolation and Characterization of Starch-utilizing Mutants of Escherichia coli
• Yeast Transformation without the Spheroplasting Process

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