Cloning and Nucleotide Sequence of the Genomic Taka-amylase A Gene of Aspergillus oryzae(Microbiology & Fermentation Industry)
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概要
- 論文の詳細を見る
The Taka-amylase A (TAA) gene was cloned from the genomic library of A. oryzae using synthetic DNA oligomers as probes. The gene was located in a 3.7 Kbp EcoRI fragment. A. oilyzae transformants containing a EcoRI fragment showed increased TAA activity, the increase being 2 to 5-fold. The complete nucleotide sequence of the gene was determined and it was found that the gene consisted of 2040 bp, with eight introns. The deduced amino acid sequence was compared with that reported by Toda et al., with the following findings. A presumed signal peptide consisting of twenty-one amino acids was found at the N-terminal. One insertion, one deletion and ten substitutions of amino acids were observed, which may be due to strain variation.
- 社団法人日本農芸化学会の論文
- 1989-03-23
著者
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Iimura Yuzuru
National Research Institute of Brewing
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Iimura Y
National Research Institute Of Brewing:(present Address) Department Of Applied Biochemistry Yamanash
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GOMI Katsuya
National Research Institute of Brewing
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TAKAHASHI Kojiro
National Research Institute of Brewing
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HARA Shodo
National Research Institute of Brewing
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YOSHIZAWA Kiyoshi
National Research Institute of Brewing
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Tsuchiya Kozo
Research Institute of Brewing Resources Co.
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TADA Setsuzo
Research Institute of Brewing Resources Co., Ltd.
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Takahashi K
Life Science Research Laboratory Japan Tobacco Inc.
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Hara S
General Research Laboratory Of Kiku-masamune Sake Brewing Co. Ltd.
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Gomi K
Tohoku Univ. Miyagi Jpn
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Tsuchiya K
Nisshin Oil Mills Ltd. Yokohama‐shi Jpn
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Yoshizawa K
Tokyo Univ. Agriculture Tokyo Jpn
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Tada Suguru
Research Institute Of Brewing Resources Co. Ltd.
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