Purification, Characterization, and Amino Acid Sequence of Foxtail Millet Trypsin Inhibitor III(Biological Chemistry)
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概要
- 論文の詳細を見る
One of the major trypsin inhibitors of foxtail millet, Setaria italica, was purified from a seed extract to an electrophoretically homogeneous protein by methods including chromatofocusing and affinity chromatography. This inhibitor (FMTI-III) was shown to be specific and single-headed for trypsin. The molecular weight and the amino acid composition together with the above nature were identical with those of another major trypsin inhibitor (FMTI-II) previously purified from foxtail millet grain. Sequence analysis of FMTI-III indicated that the protein contains 67 amino acid residues, the sequence of which is the same as that of FMTI-II except for the replacement of the C-terminal glutamine by glutamic acid. This single amino acid substitution had no effect on inhibitor-enzyme association.
- 社団法人日本農芸化学会の論文
- 1991-02-23
著者
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TAKAHASHI Kyoko
Department of Food Science and Nutrition, School of Human Environmental Sciences, Mukogawa Women’s U
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ASAO Toshio
Department of Food Science and Nutrition, School of Human Environmental Sciences, Mukogawa Women's U
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Takahashi K
Osaka Univ. Osaka
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Takahashi K
Life Science Research Laboratory Japan Tobacco Inc.
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Asao T
Mukogawa Women's Univ. Nishinomiya Jpn
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Asao Toshio
Department Of Food Science And Nutrition School Of Human Environmental Sciences Mukogawa Women'
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TASHIRO Misao
Department of Food Science and Nutrition, Faculty of Living Science, Kyoto Prefectural University
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HIRATA Chikage
Department of Food Science and Nutrition, Faculty of Living Science, Kyoto Prefectural University
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Tashiro Misao
Department Of Food Science And Nutrition Faculty Of Living Science Kyoto Prefectural University
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Hirata Chikage
Department Of Food Science And Nutrition Faculty Of Living Science Kyoto Prefectural University
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Takahashi Kyoko
Department Of Food Science And Nutrition School Of Human Environmental Sciences Mukogawa Women'
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Takahashi Kyoko
Department Of Bioproduction Science Faculty Of Horticulture Chiba University
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