Splice Isoforms of Transcription Factor Elf-1 Affecting Its Regulatory Function in Transcription-Molecular Cloning of Rat Elf-1
スポンサーリンク
概要
- 論文の詳細を見る
To elucidate the role of Elf-1 in FcεRI α chain expression, rat Elf-1 cDNAs were isolated and characterized. The rat Elf-1 cDNA of 2744 bp contained an open reading frame of 1848 bp. In addition to the full length rat Elf-1 cDNA (named type 1), two splice isoforms were isolated. One of the two isoforms lacked the amino acid residues from 85th to 120th (type 2), and the other from 85th to 175th (type 3). Similar isoforms were also observed in human tissue. Overexpression of rat Elf-1 (type 1) using a transient coexpression system inhibited of the α chain promoter activity. The inhibition activity was different between the isoforms ; the inhibition activity of type 2 was lower than that of type 1,and type 3 did not have an inhibitory effect. This observation suggested that each Elf-1 isoform played a different role in the gene expression under its control.
- 社団法人日本農芸化学会の論文
- 2000-12-23
著者
-
奥村 克純
三重大院・生資・分子細胞生物学
-
NISHIYAMA Makoto
Biotechnology Research Center, The University of Tokyo
-
Ra C
Atopy (allergy) Research Center Juntendo University School Of Medicine
-
Takahashi K
School Of Life Sciences Tokyo Univ. Of Pharmacy And Life Sciences
-
Yokota T
Foods & Pharmaceuticals Research & Development Laboratory Asahi Breweries Ltd.
-
Takahashi Kayoko
Central Research Lnstitute Meiji Milk Products Co. Ltd.
-
Nishiyama Chiharu
Department Of Agricultural Chemistry The University Of Tokyo
-
Nishiyama M
Biotechnology Research Center The University Of Tokyo
-
Nishiyama Makoto
Biotechnology Research Center The University Of Tokyo
-
Yokota Toyokazu
Foods & Pharmaceuticals Research & Development Laboratory Asahi Breweries Ltd.
-
Ra Chisei
Allergy And Atopy Research Center Juntendo University School Of Medicine
-
TAKAHASHI Kyoko
Foods & Pharmaceuticals Research & Development Laboratory, Asahi Breweries, Ltd.
-
OHTAKE Yasuyuki
Foods & Pharmaceuticals Research & Development Laboratory, Asahi Breweries, Ltd.
-
OKUMURA Ko
Allergy Research Center, Juntendo University School of Medicine
-
NISHIYAMA Chiharu
Foods and Pharmaceuticals Research and Development Laboratory, Asahi Breweries Ltd.
-
Ohtake Y
Foods & Pharmaceuticals Research & Development Laboratory Asahi Breweries Ltd.
-
Ohtake Yasuyuki
Foods And Pharmaceuticals Research And Development Laboratory Asahi Breweries Ltd.
-
Okumura Ko
Atopy (allergy) Research Center Juntendo University School Of Medicine
-
Yokota T
Faculty Of Agriculture Shizuoka University
-
Yuuki T
Asahi Breweries Ltd. Ibaraki Jpn
-
Takahashi Kyoko
Department Of Food Science And Nutrition School Of Human Environmental Sciences Mukogawa Women'
-
Kageyama T
Kyoto Univ. Aichi Jpn
-
Yokota Takao
Iss Science Project Office Institute Of Space And Astronautical Science Japan Aerospace Exploration
-
Nishiyama Makoto
Atopy (Allergy) Research Center, Juntendo University School of Medicine
関連論文
- TWEAK inhibits TGF-β-induced contraction of normal and keloid fibroblast-embedded collagen gel
- TGF-β/Smad signaling inhibits IFN-γ and TNF-α-induced TARC (CCL17) production in HaCaT cells
- 1-II-9 ニコチン酸関連化合物によるヒト白血病細胞K562の分化誘導における作用機構の検討(一般研究発表,日本ビタミン学会第61回大会研究発表要旨)
- 1-II-10 ニコチンアミドによるアポトーシスにおける形態変化抑制機構の解析(一般研究発表,日本ビタミン学会第61回大会研究発表要旨)
- 1-II-5 ニコチン酸関連化合物によるヒト慢性骨髄性白血病細胞K562の分化誘導効果について(一般研究発表,新世紀ビタミン学の展望と先進的展開を目指して,日本ビタミン学会第60回大会発表要旨)
- 1-II-4 ニコチンアミド処理によりヒト白血病細胞HL-60において発現が変動する遺伝子の解析(一般研究発表,新世紀ビタミン学の展望と先進的展開を目指して,日本ビタミン学会第60回大会発表要旨)
- NADワールドの新展開：最新のトピックスと展望
- Induction of differentiation in K562 cell line by nicotinic acid-related compounds
- Changes in the Gene Expression of C-myc and CD38 in HL-60 Cells during Differentiation Induced by Nicotinic Acid-Related Compounds
- 2-II-36 細胞死誘導時ニコチンアミド類による形態変化抑制効果に対する作用機構の解析(ビタミン学の原点・栄養学への21世紀的回帰, 日本ビタミン学会第59回大会)
- 2-II-35 ヒト白血病細胞HL-60の分化誘導過程における細胞内NADH量の解析(ビタミン学の原点・栄養学への21世紀的回帰, 日本ビタミン学会第59回大会)
- 2-II-34 白血病の分化誘導治療におけるニコチン酸関連化合物による併用効果の検討(ビタミン学の原点・栄養学への21世紀的回帰, 日本ビタミン学会第59回大会)
- 2-II-17 ヒト白血病細胞HL-60の細胞死においてニコチンアミドが制御するイベント(一般研究発表,日本ビタミン学会 第58回大会研究発表要旨)
- 2-II-16 ヒト白血病細胞HL-60の顆粒球様細胞への分化誘導後に起こる細胞死の解析(一般研究発表,日本ビタミン学会 第58回大会研究発表要旨)
- 2-II-15 ヒト白血病細胞HL-60の分化誘導における遺伝子発現制御へのニコチンアミドの関与(GGA)によるmicroRNAの発現調節(一般研究発表,日本ビタミン学会 第58回大会研究発表要旨)
- Topics:複製フォーク進行制御のダイナミクス (細胞核の世界--ダイナミクスから病態まで) -- (細胞周期と細胞分裂のダイナミクス)
- 2-B-23 ニンニクの加工処理がその機能性に及ぼす影響(第57回大会一般研究発表)(第57回大会研究発表要旨)
- 2-B-11 ニコチンアミドによるヒト白血病細胞HL-60の分化誘導過程におけるミトコンドリアの関与(第57回大会一般研究発表)(第57回大会研究発表要旨)
- 2-B-10 ヒト白血病細胞HL-60の細胞死誘導過程においてニコチンアミドによる形態変化抑制効果の作用機構(第57回大会一般研究発表)(第57回大会研究発表要旨)
- 核内クロマチン構造と複製・転写のダイナミクス
- Detection of DNA Damages Induced by Five Model Chemicals in Goldfish Carassius auratus Cells Using Comet Assay
- 2-III-4 ヒト白血病細胞においてナイアシンが細胞内NAD量およびミトコンドリアに及ぼす影響(第56回大会一般研究発表)
- 2-III-3 ヒト白血病細胞HL-60の分化誘導過程におけるポリ(ADP-リボシル)化の解析(第56回大会一般研究発表)
- 2-III-2 ヒト白血病細胞HL-60のcaspase非依存的細胞死制御機構にニコチンアミドが及ぼす影響(第56回大会一般研究発表)
- 目で見る複製・転写のダイナミクス
- Expression of CD38 in Human Promyelocytic Leukemia HL-60 Cell Line during Differentiation by Niacin-related Compounds(Biochemistry & Molecular Biology)
- Induction of Differentiation in Human Promyelocytic Leukemia HL-60 Cell Line by Niacin-related Compounds(Biochemistry & Molecular Biology)
- レプリコン説に関するEMBOワークショップ : 染色体複製から細胞分裂まで : レプリコン説40周年を記念して
- 2-II-33ピコリン酸の経口投与が動物個体に及ぼす影響について(第55回大会一般研究発表)
- 2-II-32ナイアシン関連化合物によるHL-60の分化誘導過程におけるCD38の発現(第55回大会一般研究発表)
- 2-II-31細胞死制御機構におけるニコチンアミドの役割について(第55回大会一般研究発表)
- 2-II-30Heat Shock誘導アポトーシスにおけるナイアシン関連化合物の影響について(第55回大会一般研究発表)
- クロマチン構造とDNA複製 (特集 クロマチン)
- Replication Timing Properties within the Mouse Distal Chromosome 7 Imprinting Cluster(Biochemistry & Molecular Biology)
- ナイアシン関連化合物によるHL-60の分化誘導作用および細胞内NAD量の測定
- アポトーシス誘導過程におけるNAD代謝の役割について
- アポトーシスの形態変化におよぼすニコチンアミド関連化合物の影響
- Radical Scavenging Activities of Niacin-Related Compounds(Biochemistry & Molecular Biology)
- Apoptosis induced by Picolinic Acid-related Compounds in HL-60 Cells(Biochemistry & Molecular Biology)
- Preparation of Extended Metaphase Chromosomes from Human Cultured Cells Using a Topoisomerase II Inhibitor, ICRF-193
- Visualization of Biallelic Expression of the Imprinted SNRPN Gene Induced by Inhibitors of DNA Methylation and Histone Deacetylation
- 1-II-14 フローサイトメリーによるポリ(ADP-リボシル)化の簡便・迅速な解析法
- ヒトMHC(HLA)領域に観察される巨大G+C%ドメインの構造解析
- 1-II-10 ナイアシン関連化合物がアポトーシス誘導に及ぼす影響
- 1. ユーグレナにおけるNADの生合成と代謝の経路に関する検討
- 2-II-22 過酸化水素誘導によるG_2/M期での細胞周期制御機構におけるcdc25C及び14-3-3プロテインの関与
- 2-III-23 紫外線誘導アポトーシスにおける3-アミノベンズアミドの影響について
- 1-II-12 アポトーシス誘導時における細胞内NAD量の変化の解析
- Effects of a Fermented Milk Drink Containing Lactobacillus casei Strain Shirota on the Immune System in Healthy Human Subjects
- DNA FLUORESCENCE IN SITU HYBRIDIZATION IN PARAMECIUM(Cell Biology and Morphology)Proceedings of the Seventy-First Annual Meeting of the Zoological Society of Japan
- DNA Fluorescence in situ Hybridization in Paramecium: Telomere Localization in Macronucleus : Developmental Biology
- Roles of PU.1 in monocyte- and mast cell-specific gene regulation : PU.1 transactivates CIITA pIV in cooperation with IFN-γ
- Enzyme-Linked Immunosorbent Assays with High Sensitivity for Antigen-Specific and Total Murine IgE : A Useful Tool for the Study of Allergies in Mouse Models
- Human umbilical cord epithelial cells express Notch ligands Delta1 and Jagged1
- Acne conglobata successfully treated by fractional laser after CO_2 laser abrasion of cysts combined with topical tretinoin
- IP-11 Human β-defensins and cathelicidin LL-37 on IL-31 production in human mast cells
- IP-9 Functional analysis of FCER1A-18483A>C polymorphism
- IP-7 Localization of NAD (P) H oxidase activity in allergenic pollen grains
- Benign symmetrical lipomatosis associated with alcoholism
- Protease Activity of Allergenic Pollen of Cedar, Cypress, Juniper, Birch and Ragweed
- Human umbilical cord epithelial cells express Notch1 : Implications for its epidermal-like differentiation
- Thymic stromal lymphopoietin secretion of synovial fibroblasts is positively and negatively regulated by Toll-like receptors/nuclear factor-κB pathway and interferon-γ/dexamethasone
- Combination antibody-based cancer immunotherapy
- TGF-β type I receptor kinase inhibitor down-regulates rheumatoid synoviocytes and prevents the arthritis induced by type II collagen antibody
- Human cathelicidin LL-37 increases vascular permeability in the skin via mast cell activation, and phosphorylates MAP kinases p38 and ERK in mast cells
- Synergistic effect of antibacterial agents human β-defensins, cathelicidin LL-37 and lysozyme against Staphylococcus aureus and Escherichia coli
- Inhibitory Effect of Polyphenol-Enriched Apple Extracts on Mast Cell Degranulation in Vitro Targeting the Binding between IgE and FcεRI
- GATA-1 is required for expression of FcεRI on mast cells : analysis of mast cells derived from GATA-1 knockdown mouse bone marrow
- Suppression of serum IgE response and systemic anaphylaxis in a food allergy model by orally administered high-dose TGF-β
- Critical contribution of CD80 and CD86 to induction of anterior chamber-associated immune deviation
- DNA複製開始点を標的とした新薬モニタリングシステムの構築
- 哺乳類細胞における核内ゲノムダイナミクスの可視化技術とその応用展開を目指して
- Localization of intestinal intraepithelial T lymphocytes involves regulation of α_Eβ_7 expression by transforming growth factor-β
- NMR Study on the Major Mite Allergen Der f2 : Its Refined Tertiary Structure, Epitopes for Monoclonal Antibodies and Characteristics Shared by ML Protein Group Members
- Effects of Double Mutation at Two Distant IgE-binding Sites in the Three-dimensional Structure of the Major House Dust Mite Allergen Der f 2 on IgE-binding and Histamine-releasing Activity(Biochemistry & Molecular Biology)
- Solution Structure of the SH2 Domain of Grb2 / Ash Complexed with EGF Receptor-Derived Phosphotyrosine-Containing Peptide
- Molecular Cloning of Rat Transcription Factor YY1(Biochemistry & Molecular Biology)
- Analysis of Transactivation Region of Elf-1 by Using a Yeast One-hybrid System(Biochemistry & Molecular Biology)
- Production of Humanized Antibody against Human High-affinity IgE Receptor in a Serum-free Culture of CHO Cells, and Purification of the Fab Fragments
- Molecular Cloning of Rat USF2 cDNA and Characterization of Splicing Variants
- Expression of Humanized Fab Fragments That Recognize the IgE-Binding Domain of Human FcεRIα in COS and CHO Cells
- Splice Isoforms of Transcription Factor Elf-1 Affecting Its Regulatory Function in Transcription-Molecular Cloning of Rat Elf-1
- Production of Humanized Fab Fragment against Human High Affinity IgE Receptor in Pichia pastoris
- Apoptosis Induced by Niacin-Related Compounds in K562 Cells but not in Normal Human Lymphocytes
- Apoptosis Induced by Nicotinamide-related Compounds and Quinolinic Acid in HL-60 Cells
- 1-II-11 イソニコチン酸ヒドラジド関連化合物による種々のラジカル消去活性
- 2-II-19 ニコチンアミドやキノリン酸などの関連化合物によるアポトーシス誘導及びその機構について
- Regulation of human FcεRI β chain gene expression by Oct-1
- The effect of antibacterial peptide human β-defensin-2 on interleukin-18 secretion by keratinocytes
- TRAIL and its receptors as targets for cancer therapy
- Expression and function of 4-1BB and 4-1BB ligand on murine dendritic cells
- Detection of anti-lgE and anti-FcεRI α chain auto-antibodies in patients with atopic dermatitis
- 2-II-21 ニコチン酸関連化合物のNOラジカル消去活性及び細胞内酸化ストレス状態に及ぼす影響について
- Apoptosis Induced by the Flavonoid from Lemon Fruit (Citrus limon BURM. f.) and Its Metabolites in HL-60 Cells
- Preferential expression of T_h2-type chemokine and its receptor in atopic dermatitis
- The Relationship between the Frequency of the Common Cold and the Activities of Natural Killer Cells
- P3-03 Phenotypes of marginal metallophilic macrophage and marginal zone macrophage in mouse spleen investigated by 7-color fluorescence imaging
- Direct Expression of the Extracellular Portion of Human FcεRIα Chain as Inclusion Bodies in Escherichia coli
- Epitope Analysis and Primary Structures of Variable Regions of Anti-human FcεRI Monoclonal Antibodies, and Expression of the Chimeric Antibodies Fused with Human Constant Regions
- 1-II-13 ナイアシン関連化合物によるヒト白血病細胞の分化誘導